Cloning of cDNA Coding for Guinea Pig Liver Transglutaminase(Biological Chemistry)
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概要
- 論文の詳細を見る
Seven peptides derived from guinea pig liver transglutaminase were sequenced. Using synthetic oligonucleotides that we designed from the amino acid sequences as probes, we obtained a cDNA clone of transglutaminase from a cDNA library constructed with mRNA partly purified from guinea pig liver. About 60% of the primary structure of enzyme protein was predicted by sequencing of the cDNA. The predicted amino acid sequence included a sequence of pentapeptide containing the active-site cysteine residue [J. E. Folk and P. W. Cole, J. Biol. Chem., 241, 3238 (1966)]. An expanded region of the sequence surrounding the active-site cysteine residue showed significant homology to that of thiol proteases. Two regions rich in glutamic acid residues were found; they are possible calcium-binding sites of this enzyme.
- 社団法人日本農芸化学会の論文
- 1987-03-22
著者
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IKURA Koji
Department of Applied Biology, Faculty of Textile Science, Kyoto Institute of Technology
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Ikura K
Kyoto Inst. Technol. Kyoto Jpn
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Ikura Koji
Department Of Food Science And Technology Faculty Of Agriculture Kyoto University
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Ikura Koji
Department Of Applied Biology Faculty Of Textile Science Kyoto Institute Of Technology
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Sasaki Ryuzo
Department of Food Science and Technology, Faculty of Agriculture, Kyoto University
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Yokota Hiroyuki
Department Of Emergency And Critical Care Nippon Medical School
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Yokota Hiroyuki
Department Of Food Science And Technology Faculty Of Agriculture Kyoto University
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CHIBA Hideo
Department of Food Science and Technology, Faculty of Agriculture, Kyoto University
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Chiba H
Department Of Food Science And Technology Faculty Of Agriculture Kyoto University:(present Office)la
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Sasaki Ryuzo
Department Of Food Science And Technology Faculty Of Agriculture Kyoto University
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Yokota Hiroyuki
Department Of Emergency And Critical Care Medicine Main Hospital Nippon Medical School
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Sasaki R
Department Of Food Science And Technology Faculty Of Agriculture Kyoto University
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NASU Taka-aki
Department of Food Science and Technology, Faculty of Agriculture, Kyoto University
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Nasu Taka-aki
Department Of Food Science And Technology Faculty Of Agriculture Kyoto University
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Yokota Hiroyuki
Department Of Emergency And Critical Care Medicine Graduate School Of Medicine Nippon Medical School
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YOKOTA Hiroyuki
Department of Emergency and Critical Care Medical Center, Nippon Medical School
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