Isolation and Sequence Analysis of Bovine αs1-Casein cDNA Clone
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概要
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Poly(A+) RNAwas isolated from lactating cow mammary gland and cDNAwas synthesized by a method which resulted in a high yield of products containing complete 5-terminal mRNAsequences. Transformants were screened with a HaelU-EcoRI fragment for bovine αs1-casein CDNA[M. Maki, M. Nagao, M. Hirose and H. Chiba, Agric. Biol Chem., 47, 441 (1983)]. A CDNAclone was isolated that contained the full length of αs1-casein mRNAand its nucleotide sequence was determined. The amino acid sequence predicted from the nucleotide sequence was in good agreement with that determined with the purified mature αs1-casein. The signal peptide consisting of 15 amino acids was identical to that of ovine αs1-casein. High homology at the nucleotide sequence level in the signal peptide region and in the 5-untranslated region was found between rat α-casein and bovine αs1-casein.
- 社団法人 日本農芸化学会の論文
著者
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Nagao Masaya
Department Of Food Science And Technology Faculty Of Agriculture Kyoto University:(present Office)re
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CHIBA Hideo
Department of Food Science and Technology, Faculty of Agriculture, Kyoto University
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Sasaki Ryuzo
Department Of Food Science And Technology Faculty Of Agriculture Kyoto University
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Maki Masatoshi
Department Of Applied Molecular Biological Sciences Graduate School Of Bioagricultural Sciences Nago
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Chiba Hideo
Department of Food Science and Technology, Kyoto University
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NAGAO Masaya
Department of Food Science and Technology, Kyoto University
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