The ALG-2 Binding Site in Sec31A Influences the Retention Kinetics of Sec31A at the Endoplasmic Reticulum Exit Sites as Revealed by Live-Cell Time-Lapse Imaging
スポンサーリンク
概要
- 論文の詳細を見る
ALG-2, a member of the penta-EF-hand protein family, interacts Ca2+-dependently with a COPII component, Sec31A. In this study, we first established HeLa cells stably expressing green fluorescent protein-fused ALG-2 (GFP-ALG-2) and red fluorescent protein-fused Sec31A (Sec31A-RFP). After inducing Ca2+-mobilization, the cytoplasmic distribution of GFP-ALG-2 changed from a diffuse to a punctate pattern, which extensively overlapped with the Sec31A-RFP-positive structures, indicating that ALG-2 is recruited to the endoplasmic reticulum exit sites (ERES) in living cells. Next, overlay experiments with biotin-labeled ALG-2 were done to dissect the ALG-2 binding site (ABS). They revealed that a sequence comprising amino acid residues 839–851 in the Pro-rich region was necessary and sufficient for direct binding to ALG-2. Finally, fluorescence recovery after photobleaching analysis indicated that the ABS deletion reduced the high-affinity population of Sec31A to the ERES, suggesting that the ABS is one of the key determinants of the retention kinetics of Sec31A at ERES.
- 社団法人 日本農芸化学会の論文
- 2010-09-23
著者
-
WADA Ikuo
Department of Pharmacy, Sapporo National Hospital
-
Shibata Hideki
Department of Applied Molecular Biosciences, Graduate School of Bioagricultural Sciences, Nagoya Uni
-
Inuzuka Tatsutoshi
Department of Applied Molecular Biosciences, Graduate School of Bioagricultural Sciences, Nagoya Uni
-
Yoshida Haruna
Department of Applied Molecular Biosciences, Graduate School of Bioagricultural Sciences, Nagoya Uni
-
SUGIURA Hirofumi
Department of Applied Molecular Biosciences, Graduate School of Bioagricultural Sciences, Nagoya Uni
-
MAKI Masatoshi
Department of Applied Molecular Biosciences, Graduate School of Bioagricultural Sciences, Nagoya Uni
-
Maki Masatoshi
Department Of Applied Molecular Biological Sciences Graduate School Of Bioagricultural Sciences Nago
-
Wada Ikuo
Department Of Cell Science Institute Of Biomedical Sciences Fukushima Medical University School Of M
-
YOSHTDA Haruna
Department of Applied Molecular Biosciences, Graduate School of Bioagricultural Sciences, Nagoya Uni
-
Shibata Hideki
Department Of Applied Molecular Biosciences Graduate School Of Bioagricultural Sciences Nagoya Unive
-
Wada Ikuo
Department Of Biochemistry (section Ii) Sapporo Medical University School Of Medicine
-
Yoshtda Haruna
Department Of Applied Molecular Biosciences Graduate School Of Bioagricultural Sciences Nagoya Unive
-
Sugiura Hirofumi
Department Of Applied Molecular Biosciences Graduate School Of Bioagricultural Sciences Nagoya Unive
-
Maki Masatoshi
Department Of Applied Molecular Biosciences Graduate School Of Bioagricultural Sciences Nagoya Unive
-
Inuzuka Tatsutoshi
Department Of Applied Molecular Biosciences Graduate School Of Bioagricultural Sciences Nagoya Unive
関連論文
- The ALG-2 binding site in Sec31A influences the retention kinetics of Sec31A at the endoplasmic reticulum exit sites as revealed by live-cell time-lapse imaging
- Uptake of Doxorubicin by Cultured Kidney Epithelial Cells LLC-PK_1
- Multiple small intestinal stromal tumors with skeinoid fibers in association with neurofibromatosis 1 (von Recklinghausen's disease)
- The ALG-2 Binding Site in Sec31A Influences the Retention Kinetics of Sec31A at the Endoplasmic Reticulum Exit Sites as Revealed by Live-Cell Time-Lapse Imaging
- Identification of Rab GTPase-Activating Protein-Like Protein (RabGAPLP) as a Novel Alix/AIP1-Interacting Protein
- Human Calpain 7/PalBH Associates with a Subset of ESCRT-III-related Proteins in its N-terminal Region and Partly Localizes to Endocytic Membrane Compartments
- Analyses of Expression and Localization of Two Mammalian-Type Transglutaminases in Physarum polycephalum, an Acellular Slime Mold
- The Penta-EF-Hand Protein ALG-2 Interacts with a Region Containing PxY Repeats in Alix/AIP1, Which Is Required for the Subcellular Punctate Distribution of the Amino-Terminal Truncation Form of Alix/AIP1
- Characterization of Human Recombinant Transglutaminase 1 Purified from Baculovirus-infected Insect Cells
- GTP, an Inhibitor of Transglutaminases, is Hydrolyzed by Tissue-type Transglutaminase (TGase 2) but not by Epidermal-type Transglutaminase (TGase 3)
- Characterization og Recombinant Mouse Epidermao-Type Transglutaminase (TGase3) : Regulation of Its Activity by Proteolysis and Guanine Nucleotides
- Characterization of the Trafficking Pathway of Cystic Fibrosis Transmembrane Conductance Regulator in Baby Hamster Kidney Cells
- Ultrastructural changes at the myotendinous junction induced by exercise
- Identification and Characterization of Two Penta-EF-Hand Ca^-Binding Proteins in Dictyostelium discoideum^1
- PKNβ interacts with the SH3 Domains of Graf and a Novel Graf Related Protein, Graf2, Which Are GTPase Activating Proteins for Rho Family^1
- Uninephrectomy induces progressive glomerulosclerosis and apoptosis in anti-Thy1 glomerulonephritis
- Calcium-Induced Exposure of a Hydrophobic Surface of Mouse ALG-2,Which Is a Member of the Penta-EF-Hand Protein Family
- Postnatal Development of Synovial Capillaries of Rats with Special Reference to Permeability
- A Possible Barrier Function of the Articular Surface
- Ultrastrucural Study of Cells at the Synovium-Cartilage Junction: Response of Synovial Cells of the Rat Knee Joint to Intra-Aricularly Injeted Latex Particles
- Analysis of epidermal-type transglutaminase (transglutaminase 3) in human stratified epithelia and cultured keratinocytes using monoclonal antibodies
- Cell Surface Activities of the Human Type 2b Phosphatidic Acid Phosphatase
- A Melanosome-associated Monoclonal Antibody J1Recognizes Luminal Membrane of Prelysosomes Common to Biogenesis of Melanosomes and Lysosomes
- Promotion of Tyrosinase Folding in Cos 7 Cells by Calnexin
- The ALG-2 Binding Site in Sec31A Influences the Retention Kinetics of Sec31A at the Endoplasmic Reticulum Exit Sites as Revealed by Live-Cell Time-Lapse Imaging
- Body Sway Test and Microvibration on Pregnant Women
- A Study of the Treatment of Psychosomatic Diseases in Obstetrics and Gynecology : On Fasting and Autogenic Therapy
- Early appendiceal adenocarcinoma. A review of the literature with special reference to optimal surgical procedures
- COOH-terminal isoleucine of lysosome-associated membrane protein-1 is optimal for its efficient targeting to dense secondary lysosomes
- Immunological Detection of Proteolytically Activated Epidermal-type Transglutaminase (TGase 3) Using Cleavage-site-specific Antibody(Biochemistry & Molecular Biology)
- THE CONVERSION OF EXOGENOUSLY ADMINISTERED L-DOPA TO DOPAMINE IN THE STOMACH AND LIVER OF RATS
- Cloning of cDNA Sequence Coding for Bovine αs1-Casein
- Calpain-7 binds to CHMP1B at its second α-helical region and forms a ternary complex with IST1
- Identification of Phosphorylation Sites in the C-Terminal Region of Charged Multivesicular Body Protein 1A (CHMP1A)
- Effects of Weaning and Resuckling on Casein Messenger RNA Levels in the Rat Mammary Gland
- Isolation and Sequence Analysis of Bovine αs1-Casein cDNA Clone
- Characterization of the Trafficking Pathway of Cystic Fibrosis Transmembrane Conductance Regulator in Baby Hamster Kidney Cells
- Nucleotide Sequence Comparison of mRNAs Coding for Major Calcium-sensitive Caseins between Cow and Rat
- EFFECT OF TETRODOTOXIN ON THE MAMMALIAN NEURO-MUSCULAR SYSTEM
- Synthesis of Rat Casein Antigen in Escherichia coli
- Analyses of Expression and Localization of Two Mammalian-Type Transglutaminases in Physarum polycephalum, an Acellular Slime Mold