Identification of Rab GTPase-Activating Protein-Like Protein (RabGAPLP) as a Novel Alix/AIP1-Interacting Protein

スポンサーリンク

概要

• 論文の詳細を見る

• Alix/AIP1 is a multifunctional adaptor protein involved in endocytosis, cell adhesion, and cell death. By yeast two-hybrid screening we identified a novel Alix/AIP1-interacting protein named Rab GTPase-activating protein-like protein (RabGAPLP). Interaction between Alix and RabGAPLP was confirmed by pull-down assays using fusion proteins of either glutathione-S-transferase (GST) or chitin-binding domain (CBD) and lysates of cultured mammalian cells expressing the respective proteins. Partial colocalization of FLAG-tagged RabGAPLP and green fluorescent protein (GFP)-fused Alix was observed at cell edges and filopodia-like structures by fluorescence confocal laser scanning microscopic analysis. The identity of RabGAPLP to merlin-associated protein (MAP), one of the interacting partners of neurofibromatosis type 2 (NF2) tumor suppressor gene product (merlin), implies cross-talk of membrane traffic and cell adhesion.

• 社団法人 日本農芸化学会の論文
• 2005-04-23

著者

• 柴田 秀樹

  Department Of Applied Molecular Biosciences Graduate School Of Bioagricultural Sciences Nagoya Unive

• Shibata Hideki

  Department of Applied Molecular Biosciences, Graduate School of Bioagricultural Sciences, Nagoya Uni

• MAKI Masatoshi

  Department of Applied Molecular Biosciences, Graduate School of Bioagricultural Sciences, Nagoya Uni

• Shibata Hideki

  Dep. Of Applied Molecular Biosciences Graduate School Of Bioagricultural Sciences Nagoya Univ.

• ICHIOKA Fumitaka

  Department of Applied Molecular Biosciences, Graduate School of Bioagricultural Sciences, Nagoya Uni

• HORII Mio

  Department of Applied Molecular Biosciences, Graduate School of Bioagricultural Sciences, Nagoya Uni

• KATOH Keiichi

  Department of Applied Molecular Biosciences, Graduate School of Bioagricultural Sciences, Nagoya Uni

• TERASAWA Yoshinori

  Department of Applied Molecular Biosciences, Graduate School of Bioagricultural Sciences, Nagoya Uni

• Horii Mio

  Department Of Applied Molecular Biosciences Graduate School Of Bioagricultural Sciences Nagoya Unive

• Ichioka Fumitaka

  Department Of Applied Molecular Biosciences Graduate School Of Bioagricultural Sciences Nagoya Unive

• Terasawa Yoshinori

  Department Of Applied Molecular Biosciences Graduate School Of Bioagricultural Sciences Nagoya Unive

• Katoh Keiichi

  Department Of Applied Molecular Biosciences Graduate School Of Bioagricultural Sciences Nagoya Unive

• Satoh Hirokazu

  Department Of Applied Molecular Biosciences Graduate School Of Bioagricultural Sciences Nagoya Unive

• Maki Masatoshi

  Department Of Applied Molecular Biological Sciences Graduate School Of Bioagricultural Sciences Nago

• Shibata Hideki

  Department Of Applied Molecular Biosciences Graduate School Of Bioagricultural Sciences Nagoya Unive

• Katoh Keiichi

  Department Of Anesthesiology Japanese Red Cross Medical Center

関連論文

• The ALG-2 binding site in Sec31A influences the retention kinetics of Sec31A at the endoplasmic reticulum exit sites as revealed by live-cell time-lapse imaging
• The ALG-2 Binding Site in Sec31A Influences the Retention Kinetics of Sec31A at the Endoplasmic Reticulum Exit Sites as Revealed by Live-Cell Time-Lapse Imaging
• Identification of Rab GTPase-Activating Protein-Like Protein (RabGAPLP) as a Novel Alix/AIP1-Interacting Protein
• 23pWX-6 Scanning Tunneling Microscopy Investigation of tris(phthalocyaninato)yttrium triple-decker molecules deposited on Au(111)
• Human Calpain 7/PalBH Associates with a Subset of ESCRT-III-related Proteins in its N-terminal Region and Partly Localizes to Endocytic Membrane Compartments
• Analyses of Expression and Localization of Two Mammalian-Type Transglutaminases in Physarum polycephalum, an Acellular Slime Mold
• The Penta-EF-Hand Protein ALG-2 Interacts with a Region Containing PxY Repeats in Alix/AIP1, Which Is Required for the Subcellular Punctate Distribution of the Amino-Terminal Truncation Form of Alix/AIP1
• 細胞内物質輸送の選別オルガネラMVB : エンドソームのダイナミックな膜構造の変化とその制御因子
• Characterization of Human Recombinant Transglutaminase 1 Purified from Baculovirus-infected Insect Cells
• GTP, an Inhibitor of Transglutaminases, is Hydrolyzed by Tissue-type Transglutaminase (TGase 2) but not by Epidermal-type Transglutaminase (TGase 3)
• Characterization og Recombinant Mouse Epidermao-Type Transglutaminase (TGase3) : Regulation of Its Activity by Proteolysis and Guanine Nucleotides
• Identification and Characterization of Two Penta-EF-Hand Ca^-Binding Proteins in Dictyostelium discoideum^1
• PKNβ interacts with the SH3 Domains of Graf and a Novel Graf Related Protein, Graf2, Which Are GTPase Activating Proteins for Rho Family^1
• Calcium-Induced Exposure of a Hydrophobic Surface of Mouse ALG-2,Which Is a Member of the Penta-EF-Hand Protein Family
• Analysis of epidermal-type transglutaminase (transglutaminase 3) in human stratified epithelia and cultured keratinocytes using monoclonal antibodies
• The ALG-2 Binding Site in Sec31A Influences the Retention Kinetics of Sec31A at the Endoplasmic Reticulum Exit Sites as Revealed by Live-Cell Time-Lapse Imaging
• Immunological Detection of Proteolytically Activated Epidermal-type Transglutaminase (TGase 3) Using Cleavage-site-specific Antibody(Biochemistry & Molecular Biology)
• Epithelial regeneration after diffuse alveolar damage in relation to underlying disease and DAD stage: an autopsy study
• Low-Temperature Scanning Tunneling Microscopy Investigation of Tris(phthalocyaninato)yttrium Triple-Decker Molecules Deposited on Au(111)
• Cloning of cDNA Sequence Coding for Bovine αs1-Casein
• Calpain-7 binds to CHMP1B at its second α-helical region and forms a ternary complex with IST1
• Life-threatening hemorrhagic shock after laparoscopic surgery : a case of postoperative thrombotic thrombocytopenic purpura
• Identification of Phosphorylation Sites in the C-Terminal Region of Charged Multivesicular Body Protein 1A (CHMP1A)
• Effects of Weaning and Resuckling on Casein Messenger RNA Levels in the Rat Mammary Gland
• Isolation and Sequence Analysis of Bovine αs1-Casein cDNA Clone
• Nucleotide Sequence Comparison of mRNAs Coding for Major Calcium-sensitive Caseins between Cow and Rat
• Synthesis of Rat Casein Antigen in Escherichia coli
• Analyses of Expression and Localization of Two Mammalian-Type Transglutaminases in Physarum polycephalum, an Acellular Slime Mold

もっと見る 閉じる

スポンサーリンク