Characterization of Human Recombinant Transglutaminase 1 Purified from Baculovirus-infected Insect Cells

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概要

• 論文の詳細を見る

• Transglutaminase 1 (TGase 1) is required for the formation of a cornified envelope in stratified squamous epithelia. Recombinant human TGase 1 expressed in baculovirus-infected cells was purified in a soluble form at the molecular mass of 92 kDa. Recombinant TGase 1 was susceptible to limited proteolysis by both μ-and mcalpains, the calcium-dependent intracellular cysteine proteases. Although the proteolysis did not induce the elevation of the specific enzyme activity of TGase 1,the requirement of calcium ion in the enzymatic reaction was reduced. Furthermore, the effects of GTP, nitric oxide, and sphingosylphosphocholine, known as regulatory factors for tissue-type isozyme (TGase 2), on the enzymatic activity of TGase 1 were investigated.

• 社団法人日本農芸化学会の論文
• 2000-10-23

著者

• IKURA Koji

  Department of Applied Biology, Faculty of Textile Science, Kyoto Institute of Technology

• MAKI Masatoshi

  Department of Applied Molecular Biosciences, Graduate School of Bioagricultural Sciences, Nagoya Uni

• Ikura K

  Kyoto Inst. Technol. Kyoto Jpn

• Ikura Koji

  Department Of Food Science And Technology Faculty Of Agriculture Kyoto University

• Ikura Koji

  Department Of Applied Biology Faculty Of Textile Science Kyoto Institute Of Technology

• HITOMI Kiyotaka

  Department of Applied Molecular Biosciences, Graduate School of Bioagricultural Sciences, Nagoya Uni

• Ikura K

  Department Of Applied Biology Kyoto Institute Of Technology

• YAMAGIWA Yoshihisa

  Department of Applied Molecular Biosciences, Graduate School of Bioagricultural Sciences, Nagoya Uni

• YAMANISHI Kiyofumi

  Department of Dermatology, Kyoto Prefectural University of Medicine

• Maki M

  Nagoya Univ. Nagoya Jpn

• Maki Masatoshi

  Department Of Applied Molecular Biological Sciences Graduate School Of Bioagricultural Sciences Nago

• Hitomi K

  名古屋大学大学院生命農学研究科応用分子生命科学専攻

• Yamanishi Kiyofumi

  Department Of Dermatology Hyogo College Of Medicine

• Yamanishi Kiyofumi

  Department Of Dermatology Kyoto Prefectural University Of Medicine

• Hitomi Kiyotaka

  Department Of Applied Molecular Bioscience Graduate School Of Bioagricultural Sciences Nagoya Univer

• Yamagiwa Yoshihisa

  Department Of Applied Molecular Biosciences Graduate School Of Bioagricultural Sciences Nagoya Unive

• Hitomi Kiyotaka

  Department of Applied Molecular Biological Sciences, Graduate School of Bioagricultural Sciences, Nagoya University

• HITOMI Kiyotaka

  Department of Applied Biological Sciences, School of Agriculture, Nagoya University

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