Production of Monoclonal Antibodies to Guinea Pig Liver Transglutaminase
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概要
- 論文の詳細を見る
Monoclonal antibodies are now a powerful tool in biology and medicine. Transglutaminase has been implicated in diverse biological functions, and the characteristics of its catalytic action are suitable for applied enzymology. In this study, we produced hybridoma cells which synthesize monoclonal antibodies against guinea pig liver transglutaminase by fusing mouse myeloma cells with spleen cells of mouse immunized with the enzyme protein. Eight hybridoma clones (coded 2F, 4B, 7C, 8B, 8D, 8E, 9F and 1 1C) were selected to produce monoclonal antibodies. The subclass of IgG produced by clone 9F was IgG2a and those from the seven other clones were all IgG1 The 9F antibody inhibited transglutaminase activity, but the other antibodies did not. A solid-phase antibody-binding assay showed that of these antibodies, 8D antibody has the highest affinity to the antigen. Transglutaminase protein in crude liver extract was identified with Western blotting analysis using 8Dantibody as the probe.
- 社団法人 日本農芸化学会の論文
著者
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Ikura Koji
Department Of Applied Biology Faculty Of Textile Science Kyoto Institute Of Technology
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CHIBA Hideo
Department of Food Science and Technology, Faculty of Agriculture, Kyoto University
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Sasaki Ryuzo
Department Of Food Science And Technology Faculty Of Agriculture Kyoto University
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OKUMURA Katsuzumi
Department of Bioscience, Faculty of Bioresources, Mie University
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OKUMURA Katsuzumi
Department of Food Science and Technology, Faculty of Agriculture, Kyoto University
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YANAGAWA Shin-ichi
Department of Food Science and Technology, Faculty of Agriculture, Kyoto University
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