Characterization of the Trafficking Pathway of Cystic Fibrosis Transmembrane Conductance Regulator in Baby Hamster Kidney Cells

スポンサーリンク

概要

• 論文の詳細を見る

• To examine the unknown trafficking pathway of the cystic fibrosis transmembrane conductance regulator (CFTR) from the endoplasmic reticulum (ER), we utilized baby hamster kidney cells stably expressing CFTR fused with green fluorescent protein. CFTR trafficking from the ER was visualized and analyzed by immunocytochemical analyses. Here we show that CFTR was exported from the ER to the cis-Golgi and early endosome, suggesting that CFTR transport in the early secretory pathway may utilize a non-conventional pathway. This CFTR trafficking pathway may be a target for pharmacological modulation that selectively stimulates CFTR transport.

• 公益社団法人 日本薬理学会の論文

著者

• Hashimoto Yasuaki

  Department of Hospital Pharmacy, Miki City Hospital

• Ueno Keiko

  Department Of Biochemistry And Applied Chemistry Kurume National College Of Technology

• HARADA Kazutsune

  Department of Molecular Medicine, Graduate School of Medical and Pharmaceutical Sciences, Kumamoto U

• Yamahira Kaori

  Department Of Molecular Medicine Graduate School Of Medical And Pharmaceutical Sciences Kumamoto Uni

• Kai Hirofumi

  Department Of Molecular Medicine Faculty Of Pharmaceutical Sciences Kumamoto University

• Wada Ikuo

  Department Of Biochemistry (section Ii) Sapporo Medical University School Of Medicine

• Okiyoneda Tsukasa

  Department Of Molecular Medicine Graduate School Of Medical And Pharmaceutical Sciences Kumamoto Uni

• Suico Mary

  Department Of Molecular Medicine Graduate School Of Pharmaceutical Sciences Global Coe "cell Fa

• Shuto Tsuyoshi

  Department Of Molecular Medicine Graduate School Of Pharmaceutical Sciences Global Coe "cell Fa

• WADA Ikuo

  Department of Cell Science, Institute of Biomedical Sciences, Fukushima Medical University School of Medicine

• UENO Keiko

  Department of Molecular Medicine, Graduate School of Medical and Pharmaceutical Sciences, Kumamoto University

• Kai Hirofumi

  Department of Molecular Medicine, Graduate School of Medical and Pharmaceutical Sciences, Kumamoto University

• Suico Mary

  Department of Molecular Medicine, Graduate School of Medical and Pharmaceutical Sciences, Kumamoto University

• Shuto Tsuyoshi

  Department of Molecular Medicine, Graduate School of Medical and Pharmaceutical Sciences, Kumamoto University

関連論文

• Uptake of Doxorubicin by Cultured Kidney Epithelial Cells LLC-PK_1
• Ephedrae Herba, a Component of Japanese Herbal Medicine Mao-to, Efficiently Activates the Replication of Latent Human Immunodeficiency Virus Type 1 (HIV-1) in a Monocytic Cell Line(Pharmacology)
• TLR3 induction by anticancer drugs potentiates poly I:C-induced tumor cell apoptosis
• Increased Production and/or Secretion of Pulmonary Surfactant in Rats by Long Term Sulfur Dioxide Exposure
• Glucose Uptake in Rat Skeletal Muscle L6 Cells Is Increased by Low-Intensity Electrical Current Through the Activation of the Phosphatidylinositol-3-OH Kinase (PI-3K) / Akt Pathway
• Multiple small intestinal stromal tumors with skeinoid fibers in association with neurofibromatosis 1 (von Recklinghausen's disease)
• Mild Electrical Stimulation Increases Ubiquitinated Proteins and Hsp72 in A549 Cells via Attenuation of Proteasomal Degradation
• Deterioration of Glycemic Control during Octreotide LAR Treatment in an Acromegalic Japanese Patient with Type 2 Diabetes Mellitus
• OJ-044 Genetic Variant of Cytochrome P450 2C9 in Patients Receiving Warfarin(Antithrombofic Ttherapy (H) : OJ6)(Oral Presentation (Japanese))
• The Regulation of Human β-Defensin 2 by the ETS Transcription Factor MEF (Myeloid Elf-1-Like Factor) Is Enhanced by Promyelocytic Leukemia Protein
• Bronchial asthma-related factors increasing pulmonary surfactant secretion
• Bakumondo-to (Mai-Men-Dong-Tang) increases intracellular cAMP in alveolar type II cells : Bakumondo-to stimulates production and inhibits degradation of cAMP
• Bakumondo-to (Mai-Men-Dong-Tang) increases β_1-adrenergic receptor mRNA expression in rat alveolar type II cells
• Effects of SS320A, a New Cysteine Derivative, on the Change in the Number of Goblet Cells Induced by Isoproterenolin Rat Tracheal Epithelium
• Effect of Human Neutrophil Elastase on Tracheal Mucociliary Transport in Anesthetized Quails
• Effects of Xanthine Derivatives on Phosphatidylcholine Secretion in Rat Type II Pneumocytes in the Presence of Activated Eosinophils
• Dexamethasone Increases β_2 : Adrenoceptor : Regulated Phosphatidylcholine Secretion in Rat Alveolar Type II Cells
• Effect of Leukotriene D_4 on Tracheal Mucociliary Transport Velocity in Quails
• Effects of Xanthine Derivatives on Phosphatidylcholine Secretion in Primary Culture of Rat Type II Pneumocytes
• Characterization of the Trafficking Pathway of Cystic Fibrosis Transmembrane Conductance Regulator in Baby Hamster Kidney Cells
• Inhibition of Human Tumor Cell Proliferation by the Telomerase Inhibitor TELIN
• Local Anti-inflammatory Activity and Systemic Side Effects of NM-135, a New Prodrug Glucocorticoid, in an Experimental Inflammatory Rat Model
• Ultrastructural changes at the myotendinous junction induced by exercise
• 4-AMINOPYRIDINE STIMULATES SURFACTANT SECRETION IN PRIMARY CULTURES OF ADULT RAT TYPE II PNEUMOCYTES
• Development of a new method for simultaneously evaluating mucociliaty clearance and pulmonary epithelial permeability in rabbit experiments by means of ^ FDG, three-dimensional positron emission tomography and rectilinear scan
• Steryl Glucoside is a Lipid Mediator in Stress-responsive Signal Transduction
• Uninephrectomy induces progressive glomerulosclerosis and apoptosis in anti-Thy1 glomerulonephritis
• Soluble Polyimides and Copolyimides Based on Alicyclic Dianhydride Having Cyclohexene and Tetralin Moieties
• Postnatal Development of Synovial Capillaries of Rats with Special Reference to Permeability
• A Possible Barrier Function of the Articular Surface
• Ultrastrucural Study of Cells at the Synovium-Cartilage Junction: Response of Synovial Cells of the Rat Knee Joint to Intra-Aricularly Injeted Latex Particles
• Effect of Glimepiride and Glibenclamide on S-Warfarin 7-Hydroxylation by Human Liver Microsomes, Recombinant Human CYP2C9.1 and CYP2C9.3(Biopharmacy)
• Cell Surface Activities of the Human Type 2b Phosphatidic Acid Phosphatase
• A Melanosome-associated Monoclonal Antibody J1Recognizes Luminal Membrane of Prelysosomes Common to Biogenesis of Melanosomes and Lysosomes
• The Expression of Antimicrobial Peptide Lysozyme is Increased by Treatment with Silver Nanoparticle (Atomyball S®) in Mammalian Epithelial Cells
• Promotion of Tyrosinase Folding in Cos 7 Cells by Calnexin
• The ALG-2 Binding Site in Sec31A Influences the Retention Kinetics of Sec31A at the Endoplasmic Reticulum Exit Sites as Revealed by Live-Cell Time-Lapse Imaging
• Body Sway Test and Microvibration on Pregnant Women
• A Study of the Treatment of Psychosomatic Diseases in Obstetrics and Gynecology : On Fasting and Autogenic Therapy
• Early appendiceal adenocarcinoma. A review of the literature with special reference to optimal surgical procedures
• Inhibitory Effects of Fruit Juices on Cytochrome P450 2C9 Activity in Vitro
• COOH-terminal isoleucine of lysosome-associated membrane protein-1 is optimal for its efficient targeting to dense secondary lysosomes
• THE CONVERSION OF EXOGENOUSLY ADMINISTERED L-DOPA TO DOPAMINE IN THE STOMACH AND LIVER OF RATS
• Glucose Uptake in Rat Skeletal Muscle L6 Cells Is Increased by Low-Intensity Electrical Current Through the Activation of the Phosphatidylinositol-3-OH Kinase (PI-3K)/Akt Pathway
• Synergism Between Interleukin (IL)-17 and Toll-like Receptor 2 and 4 Signals to Induce IL-8 Expression in Cystic Fibrosis Airway Epithelial Cells
• Removal of Extracellular Mg2+ Suppresses Sulfation of Glycoconjugates Secreted from Rabbit Trachea in Culture.
• Influence of corticosterone on tracheal mucociliary transport in pigeons.
• Characterization of the Trafficking Pathway of Cystic Fibrosis Transmembrane Conductance Regulator in Baby Hamster Kidney Cells
• Effects of anticholinergic bronchodilators on mucociliary transport and airway secretion.
• Protective effect of surface-active phospholipids against the acid-inducing inhibition of the tracheal mucociliary transport.
• The Regulation of Human .BETA.-Defensin 2 by the ETS Transcription Factor MEF (Myeloid Elf-1-Like Factor) Is Enhanced by Promyelocytic Leukemia Protein
• Possible Involvement of Endogenous Opioid System Located Downstream of α7 Nicotinic Acetylcholine Receptor in Mice With Physical Dependence on Nicotine
• EFFECT OF TETRODOTOXIN ON THE MAMMALIAN NEURO-MUSCULAR SYSTEM

もっと見る 閉じる

スポンサーリンク