Removal of Extracellular Mg2+ Suppresses Sulfation of Glycoconjugates Secreted from Rabbit Trachea in Culture.
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概要
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The influences of extracellular Ca<SUP>2+</SUP> and Mg<SUP>2+</SUP> concentrations on the basal secretion of glycoconjugates from rabbit trachea in organ culture were examined. Over 80% of the <SUP>35</SUP>S-labeled and [<SUP>3</SUP>H]glucosamine-labeled glycoconjugates secreted by the trachea were digested upon incubation with chondroitinase ABC. The basal secretion did not occur in the medium at 4°C, indicating an energy-dependent process. The basal secretion at 37°C of <SUP>35</SUP>S-labeled glycoconjugates was prominently suppressed in Mg<SUP>2+</SUP>-free Tyrode solution but not in Ca<SUP>2+</SUP>-free Tyrode solution containing ethyleneglycol bis(2-aminoethylether)tetraacetic acid (EGTA). In contrast, the basal secretion of [<SUP>3</SUP>H]glucosamine-labeled glycoconjugates was not affected by the Mg<SUP>2+</SUP> concentration in the medium. The results suggest that extracellular Mg<SUP>2+</SUP> largely contributes to sulfation of glycoconjugates basally secreted from rabbit trachea.
- 公益社団法人 日本薬理学会の論文
著者
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MIYATA Takeshi
Department of Pharmacological Sciences, Faculty of Pharmaceutical Sciences, Kumamoto University
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Kai Hirofumi
Department Of Molecular Medicine Faculty Of Pharmaceutical Sciences Kumamoto University
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Isohama Yoichiro
Department Of Chemico-pharmacological Sciences Graduate School Of Pharmaceutical Sciences Kumamoto U
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Miyata Takeshi
Department Of Chemico-pharmacological Sciences Graduate School Of Pharmaceutical Sciences Kumamoto U
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Kai Hirofumi
Department of Pharmacological Sciences, Faculty of Pharmaceutical Sciences, Kumamoto University
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TAKAHAMA Kazuo
Department of Chemico-Pharmacology, Faculty of Pharmaceutical Sciences, Kumamoto University
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Matsumoto Sachiko
Department of Pharmacological Sciences, Faculty of Pharmaceutical Sciences, Kumamoto University
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Kimoto Aishi
Department of Pharmacological Sciences, Faculty of Pharmaceutical Sciences, Kumamoto University
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Yoshitake Kazuhisa
Department of Pharmacological Sciences, Faculty of Pharmaceutical Sciences, Kumamoto University
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