Ovalbumin-Related Gene Y Protein Bears Carbohydrate Chains of the Ovomucoid Type
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概要
- 論文の詳細を見る
We have recently established the monoclonal antibodies (mAbs) specific to the major food allergen, ovomucoid, as mAb 7D, recognizing the carbohydrate moiety of ovomucoid, and mAb 6H, the peptide moiety (Biosci. Biothechnol. Biochem., 68, 2490–2497, (2004)). Using these mAbs, we found commercially available ovalbumin preparations contaminated with a considerable amount of ovomucoid together with other glycoproteins. To examine the contaminants, egg white was subjected to cation-exchange chromatography. An unidentified protein was found in egg white that reacted with mAb 7D but not with mAb 6H, having a molecular size of about 52 kDa and a blocked N-terminus. Two internal amino acid sequences of the fragments obtained after a lysyl endopeptidase and a hydroxylamine treatment revealed the protein to be ovalbumin Y (ovalbumin-related gene Y protein). We conclude that ovalbumin Y is a unique chimeric glycoprotein having an amino acid sequence similar to that of ovalbumin, but having a carbohydrate moiety similar to that of ovomucoid.
- 社団法人 日本農芸化学会の論文
- 2006-01-23
著者
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HIRATA Noriko
School of Pharmacy, Kinki University
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Kitabatake Naofumi
Research Institute Of Food Science Kyoto University
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Kitabatake Naofumi
Division Of Food Science And Biotechnology Graduate School Of Agriculture Kyoto University
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NARITA Hiroshi
Department of Food Science, Kyoto Women's University
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Hirata N
School Of Pharmacy Kinki University
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Hirata Noriko
Department Of Pharmaceutical Sciences Kinki University
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Hirata N
3rd Section Of Research And Development Toyo Suisan Kaisha Ltd.
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Narita H
Kyoto Women's Univ. Kyoto Jpn
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DOI Yukio
Department of Food Science, Kyoto Women's University
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HIROSE Junko
Department of Life Style Studies, School of Human Cultures, The University of Shiga Prefecture
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Kitabatake N
Division Of Food Science And Biotechnology Graduate School Of Agriculture Kyoto University
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Doi Yukio
Department Of Agricultural Chemistry Faculty Of Agriculture Shinshu University
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Doi Yukio
Department Of Food And Nutrition Kyoto Women's University
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Hirose Junko
Department Of Life Style Studies School Of Human Cultures The University Of Shiga Prefecture
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Hirose Junko
Department Of Food And Nutrition Kyoto Women's University
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Narita Hiroshi
Department Of Food And Nutrition Kyoto Women's University
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