Heating of an Ovalbumin Solution at Neutral pH and High Temperature(Food & Nutrition Sience)
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概要
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The thermal denaturation, aggregation, and degradation of hen egg white ovalbumin dissolved in distilled and deionized water (60 mg/ml, pH 7.5) was investigated by differential scanning calorimetry (DSC), polyacrylamide gel electrophoresis (PAGE), and viscosity measurement. Two independent endothermic peaks were observed up to 180℃ by the DSC analysis. The first peak appeared at around 80℃, corresponding to the denaturation temperature of ovalbumin. The second peak occurred around 140℃ due to the degradation of protein molecules as judged from the analysis by SDS-PAGE. The viscosity of the ovalbumin solution increased dramatically above 88℃ and maintained almost the same value up until heating to 140℃. The increase in viscosity after heating to 88℃ was due to the denaturation and subsequent aggregation of ovalbumin molecules as observed by SDS-PAGE. The decrease in viscosity of the samples heated above 150℃ appears to have been the result of degradation of the ovalbumin molecules.
- 社団法人日本農芸化学会の論文
- 2002-08-23
著者
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Kitabatake Naofumi
Division Of Food Science And Biotechnology Graduate School Of Agriculture Kyoto University
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Photchanachai Songsin
Division Of Food Science And Biotechnology Graduate School Of Agriculture Kyoto University
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MEHTA Alka
Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University
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Mehta Alka
Division Of Food Science And Biotechnology Graduate School Of Agriculture Kyoto University
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