Screening for Drosophila Proteins with Distinct Expression Patterns during Development by use of Monoclonal Antibodies
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概要
- 論文の詳細を見る
Kc 167 is a cell line established from Drosophila embryonic hemocytes and has been shown to express many extracellular matrix (ECM) and other proteins important during development. We have screened monoclonal antibodies (mAbs) raised against heparin affinity purified proteins from conditioned medium of Kc 167 cells to identify novel proteins with important roles for development. One mAb recognized a protein expressed with temporary and tissue specific patterns during Drosophila embryogenesis and larval development. This approach is an alternative to screening of Expression Sequence Tag (EST) clones by in situ hybridization to initiate reverse genetics. In addition, a number of mAbs recognizing ECM proteins were also identified. These mAbs will be useful for biochemical and cell biological analyses of Drosophila ECM proteins.
- 社団法人日本農芸化学会の論文
- 2000-01-23
著者
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NARITA Hiroshi
Department of Food Science, Kyoto Women's University
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Kitagawa Yasuo
Graduate School Of Bioscience Fukui Prefectural University
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Kumagai Takeshi
Graduate Program For Regulation Of Biological Signals Graduate School Of Bioagricultural Sciences Na
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HIROSE Junko
Department of Life Style Studies, School of Human Cultures, The University of Shiga Prefecture
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Kitagawa Yasuo
Graduate Program For Biochemical Regulation Graduate School Of Agricultural Sciences
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Hirose Junko
Department Of Food And Nutrition Kyoto Women's University
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Kadowaki Tatsuhiko
Graduate Program for Regulation of Biological Signals Graduate School of Bioagricultural Sciences, N
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GOTO Akira
Graduate School of Fisheries Sciences, Hokkaido University
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YOKOYAMA Hiroaki
Graduate Program for Regulation of Biological Signals, Graduate School of Bioagricultural Sciences,
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Yokoyama H
Graduate Program For Regulation Of Biological Signals Graduate School Of Bioagricultural Sciences Na
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Narita Hiroshi
Department Of Food And Nutrition Kyoto Women's University
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Kitagawa Yasuo
Graduate Program For Regulation Of Biological Signals Graduate School Of Bioagricultural Sciences Na
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Goto Akira
Graduate Program For Regulation Of Biological Signals Graduate School Of Bioagricultural Sciences Na
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Kadowaki Tatsuhiko
Graduate Program For Regulation Of Biological Signals Graduate School Of Bioagricultural Sciences Na
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