IDENTIFICATION OF HEPARIN-DEPENDENT CELL ADHESION SITE WITHIN LAMININ α4 CHAIN C-TERMINAL GLOBULAR DOMAIN

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概要

• 論文の詳細を見る

• Fibroblasts bound to α4LG1-5 and α4LG4 recombinant protein. In heparin dependent manner. Heparin blocked cell adhesion. Inhibition assays showed A4G82;TLFLAHGRLYFN and A4G83;LVFMFNVGHKKL within α4LG4 was active for cell adhesion. This site was previously identified as a heparin binding site by us. (J. Biol. Chem 2000, 275. 29458〜29465, H. Yamaguchi, H. Yamashita, Y. Kitagawa et al.) The synthetic peptide containing A4G90;GAAWKIKGPYIL also showed inhibitory activity. Stable cell lines derived from 293T cells, which express syndecan-2 or syndecan-4 were able to bind to α4LG4 recombinant proteins. These results suggest that cell adhesion to α4LG4 is mediated by cell surface heparan sulfate proteoglycans, syndecans.

著者

• 門谷 裕一

  北里大学 大学院医療系研究科 分子細胞生物学

• 門谷 裕一

  北里大学大学院 医療系研究科 分子細胞生物学

• Kitagawa Yasuo

  Graduate School Of Bioscience Fukui Prefectural University

• OKAZAKI Ikuko

  Graduate School of Environmental Earth Science, Hokkaido University

• NOMIZU Motoyoshi

  Graduate School of Environmental Earth Science, Hokkaido University

• Shinkai H

  Department Of Dermatology School Of Medicine Chiba University

• Kitagawa Yasuo

  Graduate Program For Biochemical Regulation Graduate School Of Agricultural Sciences

• Utani Atsushi

  The Department Of Dermatology Graduate School Of Medicine Chiba University

• Kuratomi Yuichiro

  Department Of Anatomy Kitasato University School Of Medicine

• 門谷 裕一

  Department Of Anatomy Kitasato University School Of Medicine

• Okazaki Ikuko

  Graduate School Of Environmental Earth Science Hokkaido University

• Kikkawa Yamato

  School Of Pharmacy Tokyo University Of Pharmacy And Life Sciences

• 野水 基義

  Tokyo University Of Pharmacy And Life Sciences

• Shinkai H

  Chiba Univ. Chiba Jpn

• Nomizu Motoyoshi

  Graduate School Of Environmental Earth Science Hokkaido University

• Matsuura Hiroshi

  The Department of Dermatology, School of medicine, Chiba University

• Suzuki Yoshiharu

  Graduate School of Environmental Earth Science, Hokkaido University

• Yamashita Yasutsune

  Graduate Course for Regulation of Biological Signals, Graduate School of Bioagricultural Sciences Bi

• Shinkai Hiroshi

  The Department of Dermatology, School of medicine, Chiba University

• Matsuura Hiroshi

  Department Of Dermatology School Of Medicine Chiba University

• Suzuki Yoshiharu

  Graduate School Of Environmental Earth Science Hokkaido University

• Yamashita Yasutsune

  Graduate Course For Regulation Of Biological Signals Graduate School Of Bioagricultural Sciences Bio

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