Expression and Chain Assembly of Human Laminin-332 in an Insect Cell-Free Translation System
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概要
- 論文の詳細を見る
Laminins are a family of large heterotrimeric glycoproteins comprising α, β, and γ chains. To determine the molecular mechanisms underlying chain assembly in vitro, we expressed human laminin-332 subunits in an insect cell-free translation system. We successfully produced the β3-γ2 heterodimer and the α3-β3-γ2 heterotrimer of the laminin coiled-coil (LCC) domain following co-translation of each chain. The α3-β3 and the α3-γ2 heterodimer were not detected, suggesting that the α3 chain can assemble with only β3-γ2 heterodimer to form a heterotrimer via disulfide bonds. These results are consistent with those of a previous report indicating that laminin chain assembly proceeds through the β-γ heterodimer to the α-β-γ heterotrimer in vivo. We suggest that the cell-free translation system is a valid system with which to study the mechanisms underlying laminin chain assembly.
- 社団法人 日本農芸化学会の論文
著者
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Kitagawa Yasuo
Graduate School Of Bioscience Fukui Prefectural University
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NIIMI Tomoaki
Graduate School of Bioagricultural Sciences, Nagoya University
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Kadowaki Tatsuhiko
Graduate Program for Regulation of Biological Signals Graduate School of Bioagricultural Sciences, N
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Ito Masaaki
Life Science Laboratory, Analytical and Measuring Instruments Division, Shimadzu Corporation
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Ezure Toru
Life Science Laboratory, Analytical and Measuring Instruments Division, Shimadzu Corporation
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Phan Hoang-phuong
Graduate School Of Bioagricultural Sciences Nagoya University
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