Preparation of N-Acylated Proteins Modified with Fatty Acids Having a Specific Chain Length Using an Insect Cell-Free Protein Synthesis System
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概要
- 論文の詳細を見る
To establish a strategy to generate N-acylated proteins modified with fatty acids having a specific chain length, tGelsolin-streptag, an epitope-tagged model protein having an N-myristoylation motif, was synthesized using an insect cell-free protein synthesis system in the presence of acyl-CoA with various fatty acid chain lengths. It was found that the fatty acid species attached to the N-termini fully depended on the acyl-CoA species added to the reaction mixture. N-Acylated proteins with fatty acid chain lengths of 8, 10, 12, and 14 were generated successfully.
- 社団法人 日本農芸化学会の論文
- 2007-01-23
著者
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Ando Eiji
Analytical And Measuring Instrument Division Shimadzu Corp.
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Ando Eiji
Life Science Laboratory Analytical & Measuring Instruments Division Shimadzu Corporation
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NISHIMURA Osamu
Life Science Laboratory, Analytical & Measuring Instruments Division, Shimadzu Corporation
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Utsumi Toshihiko
Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University
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Tsunasawa Susumu
Life Science Laboratory Analytical & Measuring Instruments Division Shimadzu Corporation
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Suzuki Takashi
Life Science Laboratory, Analytical and Measuring Instruments Division, Shimadzu Corporation
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Ito Masaaki
Life Science Laboratory, Analytical and Measuring Instruments Division, Shimadzu Corporation
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Ezure Toru
Life Science Laboratory, Analytical and Measuring Instruments Division, Shimadzu Corporation
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Shikata Masamitsu
Life Science Laboratory, Analytical and Measuring Instruments Division, Shimadzu Corporation
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Ito Masaaki
Life Science Laboratory Analytical And Measuring Instruments Division Shimadzu Corporation
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Suzuki Takashi
Analytical and Measuring Instrument Division, Shimadzu Corp.
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Utsumi Toshihiko
Appl. Mol. Biosci., Grad. Sch. Med., Yamaguchi Univ
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Utsumi Toshihiko
Department Of Biological Chemistry Faculty Of Agriculture Yamaguchi University
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Utsumi Toshihiko
Appl. Mol. Biosci. Grad. Sch. Med. Yamaguchi Univ
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Shikata Masamitsu
Life Science Laboratory Analytical And Measuring Instruments Division Shimadzu Corporation
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Nishimura Osamu
Life Science Laboratory Analytical & Measuring Instruments Division Shimadzu Corporation
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Ezure Toru
Life Sci. Lab. Analytical And Measuring Instruments Div. Shimadzu Corp.
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Nishimura Osamu
Life Science Laboratory Analytical And Measuring Instruments Division Shimadzu Corporation
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Suzuki Takashi
Analytical And Measuring Instrument Division Shimadzu Corp.
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