Characterization of Novel Cholesterol Esterase from Trichoderma sp. AS59 with High Ability to Synthesize Steryl Esters(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)

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概要

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• A novel cholesterol esterase with there and throughout to synthesisze steryl ester was obtained from the culture filtrate of a fungal strain Trichoderma sp. AS59 isolated from soil. The extra- cellular enzyme was a monomeric protein with a molecular mass of approximately 58 kDa and an isoelectric point of 4.3. The optimal temperature was between 35℃ and 40℃, and the optimal pH was 7.0. The enzyme retained 75% of the initial activity after 18 h of incubation at 30℃ in the pH range of 3.5-7.5. Its relative hydrolytic activities on fatty acid cholesteryl esters were in the following order: butyrate(121%), linoleate(100%), caprylate(79%), myristate(42%), palmitate(38%), caproate(37%), and laurate(35%). Unlike mammalian pancreatic cholesterol esterase that is activated by primary cholates on hydrolysis of long-chain fatty acid cholesteryl esters, the enzyme from Trichoderma sp. AS59 displayed its basal activity and was not affected by cholate up to a concentration of 5 mM. At higher cholate concentrations the activity gradually decreased, but reincreased at about 40 mM to reach more than twice the basal activity at 100 mM. The enzyme exhibited a broad substrate specificity, being capable of hydrolyzing various fatty acid esters of not only cholesterol, but also methanol, glycerol, and p-nitrophenol. When incubated with a mixture of cholesterol and oleic acid of equal amounts, the enzyme achieved stoichiometrical esterification in 5 h, indicating its potential utility in food additives and liquid crystal devices.

• 公益社団法人日本生物工学会の論文
• 2008-04-25

著者

• Nakayama Daisuke

  Life Science Laboratory Shimadzu Corporation

• Sugihara Akio

  Faculty Of Engineering Tokushima Bunri University

• Mizuno Takayuki

  Faculty Of Horticulture Chiba University

• Tsunasawa Susumu

  Life Science Laboratory Analytical & Measuring Instruments Division Shimadzu Corporation

• Tsunasawa Susumu

  Life Science Laboratory Shimadzu Corporation

• Maeda Atsushi

  Faculty of Engineering, Tokushima Bunri University

• Bunya Masanori

  Faculty of Engineering, Tokushima Bunri University

• Sugihara Shigeo

  Faculty of Engineering, Tokushima Bunri University

• Hirota Yoshinori

  Yashiro Co.

• Bunya Masanori

  Faculty Of Engineering Tokushima Bunri University

• Mizuno Takayuki

  Faculty Of Engineering Tokushima Bunri University

• Maeda Atsushi

  Faculty Of Engineering Tokushima Bunri University

• Sugihara Shigeo

  Faculty Of Engineering Tokushima Bunri University

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