Performance of Expression Vector, pTD1, in Insect Cell-Free Translation System(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
スポンサーリンク
概要
- 論文の詳細を見る
We constructed a pTD1 vector for an insect cell-free translation system containing a 5' untranslated region (UTR) of a polyhedrin gene as a translational enhancer sequence. Its translational efficiency was about 50-fold higher than those of mRNAs without an enhancer sequence. Moreover, the pTD1 vector functioned as an effective expression vector not only in the insect cell-free translation system but also in wheat germ extract and rabbit reticulocyte lysate systems.
- 社団法人日本生物工学会の論文
- 2006-07-25
著者
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NISHIMURA Osamu
Life Science Laboratory, Analytical & Measuring Instruments Division, Shimadzu Corporation
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Kobayashi Shinichiro
Life Science Laboratory Analytical And Measuring Instruments Division Shimadzu Corporation
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Suzuki Takashi
Life Science Laboratory, Analytical and Measuring Instruments Division, Shimadzu Corporation
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Ito Masaaki
Life Science Laboratory, Analytical and Measuring Instruments Division, Shimadzu Corporation
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Ezure Toru
Life Science Laboratory, Analytical and Measuring Instruments Division, Shimadzu Corporation
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Shikata Masamitsu
Life Science Laboratory, Analytical and Measuring Instruments Division, Shimadzu Corporation
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Tanimizu Koji
Life Science Laboratory, Analytical and Measuring Instruments Division, Shimadzu Corporation
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Ito Masaaki
Life Science Laboratory Analytical And Measuring Instruments Division Shimadzu Corporation
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Tanimizu Koji
Life Science Laboratory Analytical And Measuring Instruments Division Shimadzu Corporation
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Suzuki Takashi
Analytical and Measuring Instrument Division, Shimadzu Corp.
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Shikata Masamitsu
Life Science Laboratory Analytical And Measuring Instruments Division Shimadzu Corporation
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Nishimura Osamu
Life Science Laboratory Analytical & Measuring Instruments Division Shimadzu Corporation
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Ezure Toru
Life Sci. Lab. Analytical And Measuring Instruments Div. Shimadzu Corp.
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Nishimura Osamu
Life Science Laboratory Analytical And Measuring Instruments Division Shimadzu Corporation
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Suzuki Takashi
Analytical And Measuring Instrument Division Shimadzu Corp.
関連論文
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- Performance of Expression Vector, pTD1, in Insect Cell-Free Translation System(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
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