The Influence of Tribenoside on Expression and Deposition of Epidermal Laminins in HaCaT Cells
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概要
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Tribenoside has been used clinically for hemorrhoidal disease associated with coagulation, inflammation, and wounds. However, the pharmacological mechanism of tribenoside activity has never been clear. In this study we examined whether tribenoside affected expression and deposition of laminins that are required for reconstruction of basement membranes (BMs) during wound healing in hemorrhoidal disease. HaCaT cells, which are derived from human epidermis, were treated in growth media supplemented with tribenoside. Reverse transcriptase-polymerase chain reaction (RT-PCR) using primers specific for laminin chains showed that HaCaT cells constitutively expressed laminin α3, α5, β1, β3, γ1, and γ2 chains. Tribenoside treatment of HaCaT cells did not induce expression of other laminin chains. We also quantified the expression of laminin chains in tribenoside-treated cells using real-time PCR. The expression level of laminin α3, β1, β3, γ1, and γ2 chains was not affected. In contrast, the expression of laminin α5 in the tribenoside-treated cells was four times higher than that of control cells. Immunocytochemistry also showed that tribenoside accelerated the focal deposition of laminin-332 (α3, β3, γ2). These results suggest that tribenoside interacts with epidermal cells and regulates the expression and localization of laminins to help reconstruct BMs in wound healing of hemorrhoids.
- 公益社団法人 日本薬学会の論文
著者
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門谷 裕一
北里大学 大学院医療系研究科 分子細胞生物学
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NOMIZU Motoyoshi
Laboratory of Clinical Biochemistry, Tokyo University of Pharmacy and Life Science
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HOZUMI Kentaro
Laboratory of Bio-Material Chemistry, Division of Bioscience, Graduate School of Environmental Earth
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門谷 裕一
Department Of Anatomy Kitasato University School Of Medicine
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Hozumi Kentaro
Laboratory Of Bio-material Chemistry Division Of Bioscience Graduate School Of Environmental Earth S
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Kikkawa Yamato
School Of Pharmacy Tokyo University Of Pharmacy And Life Sciences
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MATSUDA Yuji
Laboratory of Clinical Biochemistry, Tokyo University of Pharmacy and Life Science
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KIKKAWA Yamato
Laboratory of Clinical Biochemistry, Tokyo University of Pharmacy and Life Science
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Takaki Shu
Laboratory of Clinical Biochemistry, Tokyo University of Pharmacy and Life Sciences
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Okabe Koichi
Laboratory of Clinical Biochemistry, Tokyo University of Pharmacy and Life Sciences
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Taniguchi Masakazu
Amato Pharmaceutical Products, Ltd.
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Oomachi Kengo
Amato Pharmaceutical Products, Ltd.
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Samejima Teruyuki
Amato Pharmaceutical Products, Ltd.
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Katagiri Fumihiko
Laboratory of Clinical Biochemistry, Tokyo University of Pharmacy and Life Sciences
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Nomizu Motoyoshi
Laboratory Of Clinical Biochemistry Tokyo University Of Pharmacy And Life Sciences
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Kikkawa Yamato
Laboratory Of Clinical Biochemistry Tokyo University Of Pharmacy And Life Sciences
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Katagiri Fumihiko
Laboratory Of Clinical Biochemistry Tokyo University Of Pharmacy And Life Sciences
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Kikkawa Yamato
Laboratory of Clinical Biochemistry, Tokyo University of Pharmacy and Life Sciences
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