Strategic Glycan Elution Map for the Production of Human-Type N-Linked Oligosaccharides : The Case of Hen Egg Yolk and White
スポンサーリンク
概要
- 論文の詳細を見る
Glycans play important roles in various biological phenomena, but the lack of a systematic procedure for producing complex structures of glycans severely restricts their application in the medical and industrial fields. In this paper, we propose a basic strategy for the preparation of substantial amounts (>100 mg) of N-linked oligosaccharides, where the structure of each glycan is mapped with its elution position in liquid chromatography as well as the empirical yield. In model experiments using hen egg white and yolk as starting materials, the former provided a series of agalactosylated complex-type and hybrid-type N-linked oligosaccharides containing bisecting N-acetylglucosamine (GlcNAc) in addition to two high-mannose type glycans. In contrast, egg yolk gave predominantly α2-6sialylated biantennary glycans together with a high-mannose type one, reflecting the difference in the origins of the tissues. Due to the total identity of the glycans obtained to human ones, the present strategy should provide a practical scheme for the production of human-type N-linked oligosaccharides.
- 社団法人 日本農芸化学会の論文
- 2009-03-23
著者
-
NAKAKITA Shin-ichi
Department of Chemistry, Graduate School of Science, Osaka University
-
Natsuka S
Department Of Chemistry Graduate School Of Science Osaka University
-
Hirabayashi Jun
Division Of Glyco-bioindustry Life Science Research Center Institute Of Research Promotion Kagawa Un
-
SUMIYOSHI Wataru
Division of Glyco-Bioindustry, Life Science Research Center, Institute of Research Promotion, Kagawa
-
NAKAKITA Shin-ichi
Division of Functional Glycomics, Life Science Research Center, Institute of Research Promotion, Kag
-
MIYANISHI Nobumitsu
Division of Glyco-Bioindustry, Life Science Research Center, Institute of Research Promotion, Kagawa
-
Sumiyoshi Wataru
Division Of Glyco-bioindustry Life Science Research Center Institute Of Research Promotion Kagawa Un
-
Miyanishi Nobumitsu
Division Of Glyco-bioindustry Life Science Research Center Institute Of Research Promotion Kagawa Un
-
Nakakita Shin-ichi
Division Of Functional Glycomics Life Science Research Center Institute Of Research Promotion Kagawa
関連論文
- Method for Purification of Fluorescence-Labeled Oligosaccharides by Pyridylamination(Biochemistry & Molecular Biology)
- Free Oligosaccharides with Lewis x Structure Expressed in the Segmentation Period of Zebrafish Embryo
- Alteration of Brain Type N-Glycans in Neurological Mutant Mouse Brain
- β1-4Galactosyltransferase Activity of Mouse Brain as Revealed by Analysis of Brain-Specific Complex-Type N-Linked Sugar Chains
- Development-Dependent Expression of Complex-Type Sugar Chains Specific to Mouse Brain
- Comparative Biochemical Study of N-Linked Glycans from Skin of a Squid, Todarodes pacificus
- Characterization of Wheat Germ Agglutinin Ligand on Soluble Glycoproteins in Caenorhabditis elegans
- Inhibition of Transglutaminase by Synthetic Tyrosine Melanin(Biochemistry & Molecular Biology)
- Structural Analysis of N-Linked Glycans in Caenorhabditis elegans
- High Molecular Weight Transglutaminase Inhibitor Produced by a Microorganism (Streptomyces lavendulae Y-200)
- Conversion of Pyridylamino Sugar Chains to Corresponding Reducing Sugar Chains
- The och1 Mutant of Schizosaccharomyces pombe Produces Galactosylated Core Structures of N-Linked Oligosaccharides
- Diverse Sugar-Binding Specificities of Marine Invertebrate C-Type Lectins
- A comprehensive HPLC analytical system for the identification and quantification of hexoses that employs 2-aminobenzamide coupling
- Comprehensive Analysis of N-Linked Oligosaccharides from Eggs of the Family Phasianidae
- Strategic Glycan Elution Map for the Production of Human-Type N-Linked Oligosaccharides : The Case of Hen Egg Yolk and White