Molecular Cloning and Expression of a Gene Encoding a Novel Sorbitol Oxidase from Streptomyces sp.H-7775
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概要
- 論文の詳細を見る
A gene encoding a novel intracellular sorbitol oxidase of a soil bacterium, Streptomyces sp.H-7775, was cloned and sequenced. The gene consists of an open reading frame of 1, 260-bp encoding a protein of 420 amino acids with a molecular weight of 45, 148. Deduced amino acid sequence of the gene has 25.3% identity and 68.1% similarity to that of rat L-gulonolactone oxidase at the overall amino acids. Nucleotide-binding motifs were not found in the deduced amino acid sequence of SOX protein. We succeeded in expressing recombinant sorbitol oxidase with covalently bound FAD in E. coli at about a 4, 000-fold higher total enzyme activity than that of the Streptomyces sp. H-7775. The enzymatic properties of the recombinant SOX were similar to those of the enzyme from Streptomyces sp.H-7775. This is the first report of the cloning and expression of a newly categorized enzyme, sorbitol oxidase, from Streptomyces sp.
- 社団法人日本農芸化学会の論文
- 1998-02-23
著者
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Hiraga Kazumi
Department of Applied Biology, Kyoto Institute of Technology
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ODA Kohei
Department of Applied Biology, Kyoto Institute of Technology
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Hiraga Kazumi
Department Of Applied Biology Faculty Of Textile Science Kyoto Institute Of Technology
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Oda Kohei
Department Of Applied Biology Faculty Of Textile Science Kyoto Institute Of Technology
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Oda Kohei
Department Of Agricultural Chemistry College Of Agriculture University Of Osaka Prefecture
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Eto Takashi
Asahi Chemical Industry
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YOSHIOKA Issei
Asahi Chemical Industry
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YOSHIOKA Issei
Diagnostics Research and Development Department, Asahi-Kasei Corporation
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Yoshioka I
Diagnostics Research And Development Department Asahi-kasei Corporation
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