A Novel Glycerol Kinase from Flavobacterium meningosepticum : Characterization, Gene Cloning and Primary Structure

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概要

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• A thermostable glycerol kinase (FGK) was purified 34-fold to homogeneity from Flavobacterium meningosepticum. The molecular masses of the enzyme were 200 kDa by gel filtration and 50 kDa by SDS-PAGE. The Km for glycerol and ATP were 0.088 and 0.030 mM, respectively. The enzyme was stable at 65℃ for 10 min and at 37℃ for two weeks. The enzyme gene was cloned into Escherichia coli and its complete DNA was sequenced. The FGK gene consists of an open reading frame of 1494-bp encoding a protein of 498 amino acids. The deduced amino acid sequence of the gene had 40-60% similarity to those of glycerol kinases from other origins and the amino acid sequence of the putative active site residue reported for E. coli GK is identical to the corresponding sequence of FGK except for one amino acid residue.

• 社団法人日本農芸化学会の論文
• 1998-12-23

著者

• OHSHIMA TOSHIHISA

  Department of Chemistry, Kyoto University of Education

• MATSUMOTO Kunio

  Department of Tumor Dynamics and Regulation, Cancer Research Institute, Kanazawa University

• SAKASEGAWA SHIN-ICHI

  Diagnostics Division, Asahi Kasei Corporation

• MISAKI HIDEO

  Diagnostics Division, Asahi Kasei Corporation

• Matsumoto Kunio

  Department Of Applied Chemistry Faculty Of Engineering Kanagawa Institute Of Technology

• Takahashi M

  Diagnostics Research And Development Department Asahi-kasei Corporation

• Misaki Hideo

  Diagnostics Division Asahi Kasei Corporation

• Misaki Hideo

  Asahi Chemical Industry Co. Ltd.

• YOSHIOKA Issei

  Asahi Chemical Industry

• KOGA Shinji

  Diagnostics Research and Development Department, Asahi-Kasei Corporation

• YOSHIOKA Issei

  Diagnostics Research and Development Department, Asahi-Kasei Corporation

• SAKASEGAWA Shinichi

  Asahi Chemical Industry Co. Ltd.

• KOGA Shinzi

  Asahi Chemical Industry Co. Ltd.

• TAKAHASHI Mamoru

  Asahi Chemical Industry Co. Ltd.

• SAKASEGAWA Shin-ichi

  Asahi Kasei Corporation

• Yoshioka I

  Diagnostics Research And Development Department Asahi-kasei Corporation

• Koga S

  Diagnostics Research And Development Department Asahi-kasei Corporation

• Ohshima T

  Microbial Genetics Division Institute Of Genetic Resources Faculty Of Agriculture Kyushu University

• Ohshima Toshihisa

  Department Of Biological Science And Technology Faculty Of Engineering The University Of Tokushima

• Sakasegawa Shin-ichi

  Diagnostics Division Asahi Kasei Corporation

• Matsumoto Kunio

  Department Of Applied Chemistry Kanagawa Institute Of Technology

• Misaki H

  Asahi Kasei Corporation

• Ohshima Toshihisa

  Microbial Genetics Division, Institute of Genetic Resources, Faculty of Agriculture, Kyushu University

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