Isolation of a Fosfomycin-Hypersensitive Mutant and Production of α-D-Glucose-1-Phosphate from Bacillus sp.BA-3796 Screened by Its Use

概要

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The development of a very sensitive and highly specific screening method for detection of new cell wall inhibitors of the fosfomycin type is described. A fosfomycin-hypersensitive mutant, f-360,was isolated from Staphylococcus aureus Newman by selection with fosfomycin, an antibiotic that inhibits synthesis of the bacterial cell wall. The mutant f-360 was 50-fold more sensitive tnan the parent strain to fosfomycin. The mutant was constitutive for the hexose phosphate transport system. Using this organism in screening, BA-3796,which had an antibacterial activity avainst mutant f-360 was found to be produced by a bacterium designated Bacillus sp. BA-3796. Starch and beef extract were the most suitable carbon and nitrogen sources for BA-3796 production and the amount of BA-3796 reached 3 g/l at a maximum level. The purified BA-3796 was identified as α-D-glucose-1-phosphate by its various physiochemical properties.α-D-Glucose-1-phosphate showed an antibacterial activity against Staphylococci in the presence of a slight amount of α-D-glucose-6-phosphate.
社団法人日本生物工学会の論文
1988-12-25