Stable Ammonia-specific NAD Synthetase from Bacillus stearothermophilus : Purification, Characterization, Gene Cloning, and Applications(Microbiology & Fermentation Technology)

スポンサーリンク

概要

• 論文の詳細を見る

• Bacillus stearothermophilus H-804 isolated from a hot spring in Beppu, Japan, produced an ammonia-specific NAD synthetase (EC 6. 3. 1. 5). The enzyme specifically used NH_3 as an amide donor for the synthesis of NAD as it formed AMP and pyrophosphate from deamide-NAD and ATP. None of the l-amino acids tested, such as l-asparagine or l-glutamine, or other amino compounds such as urea, uric acid, or creatinine was used instead of NH_3. Mg^<2+> was needed for the activity, and the maximum enzyme activity was obtained with 3 mM MgCl_2. The molecular mass of the native enzyme was 50 kDa by gel filtration, and SDS-PAGE showed a single protein band at the molecular mass of 25 kDa. The optimum pH and temperature for the activity were from 9.0 to 10.0 and 60℃, respectively. The enzyme was stable at a pH range of 7.5 to 9.0 and up to 60℃. The K_m for NH_3, ATP, and deamide-NAD were 0.91, 0.052, and 0.028 mM, respectively. The gene encoding the enzyme consisted of an open reading frame of 738 bp and encoded a protein of 246 amino acid residues. The deduced amino acid sequence of the gene had about 32% homology to those of Escherichia coli and Bacillus subtilis NAD synthetases. We caused the NAD synthetase gene to be expressed in E. coli at a high level ; the enzyme activity (per liter of medium) produced by the recombinant E. coli was 180-fold that of B. stearothermophilus H-804. The specific assay of ammonia and ATP (up to 25 μM) with this stable NAD synthetase was possible.

• 社団法人日本農芸化学会の論文
• 2002-10-23

著者

• OHSHIMA TOSHIHISA

  Department of Chemistry, Kyoto University of Education

• Sakuraba H

  Department Of Analytical Biochemistry Meiji Pharmaceutical University

• SAKURABA Haruhiko

  Department of Applied Biological Science, Faculty of Agriculture & Graduate School of Agriculture, K

• Takahashi M

  Diagnostics Research And Development Department Asahi-kasei Corporation

• YAMAGUCHI Fumihiko

  Planova Technology Development, Asahi-Kasei Corporation

• KOGA Shinji

  Diagnostics Research and Development Department, Asahi-Kasei Corporation

• YOSHIOKA Issei

  Diagnostics Research and Development Department, Asahi-Kasei Corporation

• TAKAHASHI Mamoru

  Diagnostics Research and Development Department, Asahi-Kasei Corporation

• Sakuraba Haruhiko

  Department Of Agricultural Chemistry University Of Osaka Prefecture

• Ohshima T

  Department Of Food Science And Technology Tokyo University Of Fisheries

• Yoshioka I

  Diagnostics Research And Development Department Asahi-kasei Corporation

• Yoshioka Issei

  Diagnostics Division Asahi Chemical Industry

• Koga S

  Diagnostics Research And Development Department Asahi-kasei Corporation

• Ohshima T

  Microbial Genetics Division Institute Of Genetic Resources Faculty Of Agriculture Kyushu University

• Ohshima Toshihisa

  Department Of Biological Science And Technology Faculty Of Engineering The University Of Tokushima

• Koga Shinji

  Diagnostics R&d Department Fine Chemicals And Diagnostics Division Asahi Kasei Pharma Corporatio

• Takahashi Mamoru

  Diagnostics Division Asahi Chemical Industry

• Yamaguchi Fumihiko

  Planova Technology Development Asahi-kasei Corporation

• Ohshima Toshihisa

  Microbial Genetics Division, Institute of Genetic Resources, Faculty of Agriculture, Kyushu University

関連論文

• Preparation of Optically Active α-Hydroxy Esters : Stereoselective Reduction of α-Keto Esters Using Thermophilic Actinomycetes
• Stereoselective Reduction of α- and β-Keto Esters with Aerobic Thermophiles, Bacillus Strains
• Cooperation of Photosynthetic Reaction Center of Photosystem II under Weak Light as Shown by Light Intensity-dependence of the Half-effective Dose of Atrazine
• Isolation of the D1 Protein and a 28-kDa Fragment of the D2 Protein from Spinach Photosystem II Membranes
• Accumulation of cholesterol and G_ ganglioside in cells cultured in the presence of progesterone:an implication for the basic defect in Niemann-Pick disease typc C
• Peptide Synthesis in Aqueous Solution. V.^ Properties and Reactivities of (p-Hydroxyphenyl)benzylmethylsulfonium Salts for Direct Benzyl Esterification of N-Acylpeptides^
• Microbial Serine Carboxypeptidase Inhibitors : Comparative Analysis of Actions on Homologous Enzymes Derived from Man, Yeast and Wheat
• Novel missense mutations in the human lysosomal sialidase gene in sialidosis patients and prediction of structural alterations of mutant enzymes
• Molecular and structural studies of Japanese patients with sialidosis type 1
• Structural basis of the GM2 gangliosidosis B variant
• Comparison of the effects of agalsidase alfa and agalsidase beta on cultured human Fabry fibroblasts and Fabry mice
• Clinical, biochemical, and cytochemical studies on a Japanese Salla disease case associated with a renal disorder
• An Amperometric D-Amino Acid Biosensor Prepared with a Thermostable D-Proline Dehydrogenase and a Carbon Nanotube-Ionic Liquid Gel
• Uptake of a Recombinant Human α-L-Iduronidase (laronidase) by Cultured Fibroblasts and Osteoblasts(Molecular and Cell Biology)
• Establishment of immortalized Schwann cells from Fabry mice and their low uptake of recombinant α-galactosidase
• Corrective effect on Fabry mice of yeast recombinant human α-galactosidase with N-linked sugar chains suitable for lysosomal delivery
• Electron microscopical and immunohistochemical studies on the localization of cathepsin B, D, L, LAMP-1 and μ-calpain in developing hair follicles
• Localization of Cathepsins B, D, L, LAMP-1 and μ-Calpain in Developing Hair Follicles
• P-10 Localization of Cathepsin B and μ Calpain in Hair Follicles of Neonatal Rats.
• Structural and biochemical studies on Pompe disease and a "pseudodeficiency of acid α-glucosidase"
• Three dimensional structural studies of α-N-acetylgalactosaminidase (α-NAGA) in α-NAGA deficiency (Kanzaki disease) : different gene mutations cause peculiar structural changes in α-NAGAs resulting in different substrate specificities and clinical phenoty
• II-C-21 Morphological analysis of the effects of enzyme replacement therapy for Fabry model mice(THE 45TH ANNUAL MEETING OF THE JAPAN SOCIETY OF HISTOCHEMISTRY AND CYTOCHEMISTRY)
• Structural and immunocytochemical studies on α-N-acetylgalactosaminidase deficiency (Schindler/Kanzaki disease)
• Molecular and structural studies of the GM2 gangliosidosis O variant
• Structural and functional study of K453E mutant protective protein/cathepsin A causing the late infantile form of galactosialidosis
• Fabry disease in patients receiving maintenance dialysis
• High Incidence of Thrombosis in Fabry's Disease
• Molecular form and subcellular distribution of acid β-galactosidase in fibroblasts from patients with G_ gangliosidosis,Morquio B disease and galactosialidosis
• Screening and detection of gene mutations in Japanese patients with Fabry disease by non-radioactive single-stranded conformation polymorphism analysis
• TWO NOVEL MUTATIONS IN THE α-GALACTOSIDASE GENE IN JAPANESE CLASSICAL HEMIZYGOTES WITH FABRY DISEASE
• Evidence for Direct Binding of Intracellularly Distributed Ganglioside GM2 to Isolated Vimentin Intermediate Filaments in Normal and Tay-Sachs Disease Human Fibroblasts
• Urinary excretion of the vitron receptor (integrin αυβ_3) in patients with non-insulin dependent diabetes mellitus
• 1A16-2 Crystallization and preliminary X-ray diffraction analysis of L-aspartate oxidase from the hyperthermophilic archaeon, Pyrococcus furiosus
• Inhibitory effect of enokitake extract on melanosis of shrimp
• Molecular Cloning and Expression of a Gene Encoding a Novel Sorbitol Oxidase from Streptomyces sp.H-7775
• Stable Ammonia-specific NAD Synthetase from Bacillus stearothermophilus : Purification, Characterization, Gene Cloning, and Applications(Microbiology & Fermentation Technology)
• Biochemical Characterizatio, Cloning, and Sequencing of ADP-Dependent (AMP-Forming) Glucokinase from Two Hyperthermophilic Archaea, Pyrococcus furiosus and Thermococcus litoralis^1
• A Novel Glycerol Kinase from Flavobacterium meningosepticum : Characterization, Gene Cloning and Primary Structure
• Establishment of Radioimmunoassay for Human Macrophage Colony-stimulating Factor Using Recombinant Human Macrophage Colony-stimulating Factor as Tracer and Immunogen
• Purification and Characterization of Recombinant Human Macrophage Colony-stimulating Factor Expressed in Chinese Hamster Ovary Cells
• A Novel Flavin Adenine Dinucleotide(FAD) Containing D-Lactate Dehydrogenase from the Thermoacidophilic Crenarchaeota Sulfolobus tokodaii Strain 7 : Purification, Characterization and Expression in Escherichia coli(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZY
• Subcellular Localization and Some Properties of NADP^+ -Linked Malic Enzymes in Euglena
• Separation and Determination of Adenine Nucleotides by High-Performance Liquid Chromatography(Analytical Chemistry)
• Fructose-2,6-bisphosphate Activates Pyruvate Kinase from the Protist Euglena gracilis
• Transcriptional Regulation of Phosphoenolpyruvate Synthase by Maltose in the Hyperthermophilic Archaeon, Pyrococcus furiosus
• A Novel Chemoattractant Lectin, Karatoxin, From the Dorsal Spines of the Small Scorpionfish Hypodytes rubripinnis
• Purification and Properties of Extremely Thermostable Glutamate Dehydrogenases from Two Hyperthermophilic Archaebacteria, Pyrococcus woesei and Pyrococcus furiosus
• Stabilization of Flavobacterium meningosepticum Glycerol Kinase by Introduction of a Hydrogen Bond(Biochemistry & Molecular Biology)
• Isolation of a Fosfomycin-Hypersensitive Mutant and Production of α-D-Glucose-1-Phosphate from Bacillus sp.BA-3796 Screened by Its Use
• Structural characterization of mutant α-galactosidases causing Fabry disease
• Purification and Some Properties of Isocitrate Lyase from Paecilomyces varioti with High Malic Acid Production
• Oxidative Stress Response in an Anaerobic Hyperthermophilic Archaeon : Presence of a Functional Peroxiredoxin in Pyrococcus horikoshii
• Cloning, Expression, and Characterization of the First Archaeal ATP-Dependent Glucokinase from Aerobic Hyperthermophilic Archaeon Aeropyrum pernix
• Extinction of Organelles in Differentiating Epidermis
• The Unique Kinetic Behavior of the Very Large NAD-Dependent Glutamate Dehydrogenase from Janthinobacterium lividum
• Fast thermal denaturation of the hyperthermophilic protein, ST1625 product
• Alteration of Substrate Specificity of Fructosyl-Amino Acid Oxidase from Ulocladium sp. JS-103(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
• Structural basis of neuronal ceroid lipofuscinosis 1
• Three-dimensional brain visualization for metachromatic leukodystrophy
• Novel Energy Metabolism in Anaerobic Hyperthermophilic Archaea : a Modified Embden-Meyerhof Pathway
• Instability of Quasi-Linear Hyperbolic Systems : The Hamiltonian Dynamical System Approach
• Temperature Dependence of Kinetic Parameters for Hyperthermophilic Glutamate Dehydrogenase from Aeropyrum pernix K1(Biochemistry & Molecular Biology)
• A Novel Chemoattractant Lectin, Karatoxin, From the Dorsal Spines of the Small Scorpionfish Hypodytes rubripinnis
• Identification and Characterization of UDP-Glucose Dehydrogenase from the Hyperthermophilic Archaon, Pyrobaculum islandicum
• On Ergodicity in 3D Closed Billiards
• The Inverse Problem of Flobenius-Perron Equations in 1D Difference Systems : 1D Map Idealization : General and Mathematical Physics
• Phase Description Method to Invariant Densities in 1-D Difference Systems : General and Mathematical Physics
• Stably Propagating Periodic Waves in Intrinsically Bistable Reaction-Diffusion Systems
• Obliquely Colliding Waves in Reaction-Diffusion Systems : Condensed Matter and Statistical Physics
• Scaling Laws of Moments in Sinai's Billiard Systems. I : The Case of Rectangular Lattice : Condensed Matter and Statistical Physics
• Schrodinger Equation Approach to Collision Phenomena in Reaction-Diffusion Systems : Condensed Matter and Statistical Physics
• Global Scaling Laws in 3D Sinai's Billiards
• Localized Patterns in Reaction-Diffusion Systems
• Rotating Spiral Waves in Reaction-Diffusion Systems : Phase Singularities of Multi-Armed Waves
• Identification and Characterization of UDP-Glucose Dehydrogenase from the Hyperthermophilic Archaon, Pyrobaculum islandicum
• Cloning and Sequence Analysis of the Gene for Phosphoenolpyruvate Carboxylase from an Extreme Thermophile, Thermus sp
• Gene Expression and Characterization of a Third Type of Dye-Linked L-Proline Dehydrogenase from the Aerobic Hyperthermophilic Archaeon, Aeropyrum pernix
• Schrodinger Equation Approach to Rotating Spiral Waves in Reaction-Diffusion Systems
• Phase Description Method to Time Averages in dissipative Chaos : Construction of Modified Maps : General and Mathematical Physics
• Phase Description Method to Time Averages in the Lorenz System : General and Mathematical Physics
• Structural Characteristics of Active and Inactive Glutamate Dehydrogenases from the Hyperthermophile Pyrobaculum islandicum
• Fabrication and Characterization of a Thermostable Quinoprotein Aldose Sugar Dehydrogenase Immobilized Electrode
• Modification of leucine dehydrogenase by pyridoxal 5'-phosphate.
• Oxidative Stress Response in an Anaerobic Hyperthermophilic Archaeon: Presence of a Functional Peroxiredoxin in Pyrococcus horikoshii
• Cloning, Expression, and Characterization of the First Archaeal ATR-Dependent Glucokinase from Aerobic Hyperthermophilic Archaeon Aeropyrum pernix

もっと見る 閉じる

スポンサーリンク