Transcriptional Regulation of Phosphoenolpyruvate Synthase by Maltose in the Hyperthermophilic Archaeon, Pyrococcus furiosus
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概要
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The phosphoenolpyruvate synthase (EC 2.7.9.2) from the hyperthermophilic archaeon Pyrococcus furiosus catalyzes the Pi-dependent formation of pyruvate, ATP and water from phosphoenolpyruvate and AMP [Sakuraba, H.et al., Arch.Biochem.Biophys., 364, 125-128 (1999)]. In this study, the P.furiosus phosphoenolpyruvate synthase was purified to homogeneity and the N-terminal amino acid sequence was determined to be AYRFIKWFEELS. The sequence coincided completely with the N-terminal amino acid sequence of the translation product of the mlrA gene that was found to be upregulated at the transcriptional level by the α-linked glucose disaccharide maltose [Robinson, K.A.and Schreier, H.J., Gene, 151, 173-176 (1994)]. The mlrA gene was cloned and expressed in Escherichia coli. The recombinant cells produced a hyperthermostable phosphoenolpyruvate synthase. This indicates that the mlrA gene encodes the enzyme. When P.furiosus was grown on the medium supplemented with maltose, the specific activity of the enzyme markedly increased (about 10-fold) compared with that produced by the cells grown on the medium without maltose. Northern blot analysis revealed enhanced transcription of the mlrA gene in the presence of maltose. These results indicate that transcriptional regulation of phosphoenolpyruvate synthase by maltose is present in P.furiosus.
- 社団法人日本生物工学会の論文
- 2001-08-25
著者
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OHSHIMA TOSHIHISA
Department of Chemistry, Kyoto University of Education
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SAKURABA Haruhiko
Department of Applied Biological Science, Faculty of Agriculture & Graduate School of Agriculture, K
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Schreier Harold
Center Of Marine Biotechnology University Of Maryland Biotechnology Institute Columbus Center
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Sakuraba Haruhiko
Department Of Agricultural Chemistry University Of Osaka Prefecture
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Sakuraba Haruhiko
Department Of Biological Science And Technology Faculty Of Engineering The University Of Tokushima
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UTSUMI EMI
Department of Biological Science and Technology, Faculty of Engineering, The University of Tokushima
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Ohshima T
Department Of Food Science And Technology Tokyo University Of Fisheries
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Utsumi Emi
Department Of Biological Science And Technology Faculty Of Engineering The University Of Tokushima
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Ohshima Toshihisa
Department Of Biological Science And Technology Faculty Of Engineering The University Of Tokushima
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