Identification and Characterization of UDP-Glucose Dehydrogenase from the Hyperthermophilic Archaon, Pyrobaculum islandicum

スポンサーリンク

概要

• 論文の詳細を見る

• A gene encoding a UDP-glucose dehydrogenase homologue was identified in the hyperthermophilic archaeon, Pyrobaculum islandicum. This gene was expressed in Escherichia coli, and the product was purified and characterized. The expressed enzyme is the most thermostable UDP-glucose dehydrogenase so far described, with a half-life of 10 min at 90 °C. The enzyme retained its full activity after incubating in a pH range of 5.0–10.0 for 10 min at 80 °C. The temperature dependence of the kinetic parameters for this enzyme was examined at 37–70 °C. A decrease in Kms for UDP-glucose and NAD was observed with decreasing temperature. This resulted in the enzyme still retaining high catalytic efficiency (Vmax⁄Km) for the substrate and cofactor, even at 37 °C. These characteristics make the enzyme potentially useful for its application at a much lower temperature such as 37 °C than the optimum growth temperature of 100 °C for P. islandicum.

著者

• Sakuraba Haruhiko

  Department Of Agricultural Chemistry University Of Osaka Prefecture

• Satomura Takenori

  Department Of Materials Science Yonago National College Of Technology

• Ohshima T

  Microbial Genetics Division Institute Of Genetic Resources Faculty Of Agriculture Kyushu University

• Ohshima Toshihisa

  Microbial Genetics Division Institute Of Genetic Resources Faculty Of Agriculture Kyushu University

• KUSUMI Kaori

  Department of Applied Biological Science, Faculty of Agriculture, Kagawa University

関連論文

• Preparation of Optically Active α-Hydroxy Esters : Stereoselective Reduction of α-Keto Esters Using Thermophilic Actinomycetes
• Stereoselective Reduction of α- and β-Keto Esters with Aerobic Thermophiles, Bacillus Strains
• An Amperometric D-Amino Acid Biosensor Prepared with a Thermostable D-Proline Dehydrogenase and a Carbon Nanotube-Ionic Liquid Gel
• 1A16-2 Crystallization and preliminary X-ray diffraction analysis of L-aspartate oxidase from the hyperthermophilic archaeon, Pyrococcus furiosus
• Stable Ammonia-specific NAD Synthetase from Bacillus stearothermophilus : Purification, Characterization, Gene Cloning, and Applications(Microbiology & Fermentation Technology)
• Biochemical Characterizatio, Cloning, and Sequencing of ADP-Dependent (AMP-Forming) Glucokinase from Two Hyperthermophilic Archaea, Pyrococcus furiosus and Thermococcus litoralis^1
• A Novel Glycerol Kinase from Flavobacterium meningosepticum : Characterization, Gene Cloning and Primary Structure
• A Novel Flavin Adenine Dinucleotide(FAD) Containing D-Lactate Dehydrogenase from the Thermoacidophilic Crenarchaeota Sulfolobus tokodaii Strain 7 : Purification, Characterization and Expression in Escherichia coli(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZY
• Subcellular Localization and Some Properties of NADP^+ -Linked Malic Enzymes in Euglena
• Separation and Determination of Adenine Nucleotides by High-Performance Liquid Chromatography(Analytical Chemistry)
• Fructose-2,6-bisphosphate Activates Pyruvate Kinase from the Protist Euglena gracilis
• Transcriptional Regulation of Phosphoenolpyruvate Synthase by Maltose in the Hyperthermophilic Archaeon, Pyrococcus furiosus
• A Novel Chemoattractant Lectin, Karatoxin, From the Dorsal Spines of the Small Scorpionfish Hypodytes rubripinnis
• Purification and Properties of Extremely Thermostable Glutamate Dehydrogenases from Two Hyperthermophilic Archaebacteria, Pyrococcus woesei and Pyrococcus furiosus
• Stabilization of Flavobacterium meningosepticum Glycerol Kinase by Introduction of a Hydrogen Bond(Biochemistry & Molecular Biology)
• Oxidative Stress Response in an Anaerobic Hyperthermophilic Archaeon : Presence of a Functional Peroxiredoxin in Pyrococcus horikoshii
• Cloning, Expression, and Characterization of the First Archaeal ATP-Dependent Glucokinase from Aerobic Hyperthermophilic Archaeon Aeropyrum pernix
• The Unique Kinetic Behavior of the Very Large NAD-Dependent Glutamate Dehydrogenase from Janthinobacterium lividum
• Fast thermal denaturation of the hyperthermophilic protein, ST1625 product
• Kenji Soda-researching enzymes with the spirit of an alpinist
• Novel Energy Metabolism in Anaerobic Hyperthermophilic Archaea : a Modified Embden-Meyerhof Pathway
• Temperature Dependence of Kinetic Parameters for Hyperthermophilic Glutamate Dehydrogenase from Aeropyrum pernix K1(Biochemistry & Molecular Biology)
• A Novel Chemoattractant Lectin, Karatoxin, From the Dorsal Spines of the Small Scorpionfish Hypodytes rubripinnis
• Identification and Characterization of UDP-Glucose Dehydrogenase from the Hyperthermophilic Archaon, Pyrobaculum islandicum
• Amino acid components of lees in salmon fish sauce are tyrosine and phenylalanine(BREWING AND FOOD TECHNOLOGY)
• Identification and Characterization of UDP-Glucose Dehydrogenase from the Hyperthermophilic Archaon, Pyrobaculum islandicum
• Gene Expression and Characterization of a Third Type of Dye-Linked L-Proline Dehydrogenase from the Aerobic Hyperthermophilic Archaeon, Aeropyrum pernix
• Gene Expression and Characterization of a Third Type of Dye-Linked L-Proline Dehydrogenase from the Aerobic Hyperthermophilic Archaeon, Aeropyrum pernix
• Fabrication and Characterization of a Thermostable Quinoprotein Aldose Sugar Dehydrogenase Immobilized Electrode
• Oxidative Stress Response in an Anaerobic Hyperthermophilic Archaeon: Presence of a Functional Peroxiredoxin in Pyrococcus horikoshii
• Cloning, Expression, and Characterization of the First Archaeal ATR-Dependent Glucokinase from Aerobic Hyperthermophilic Archaeon Aeropyrum pernix

もっと見る 閉じる

スポンサーリンク