Localization of Cathepsins B, D, L, LAMP-1 and μ-Calpain in Developing Hair Follicles

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概要

• 論文の詳細を見る

• Hair follicles consist of multilayers encircling the core hair shaft, in which the hair cortex is surrounded by the hair cuticle. The encircling layers are broadly classified into internal and external root sheaths. The internal root sheath consists of cuticles of internal root sheath, Huxley's layer and Henle's layer. A layer lies between the external root sheath and Henle's layer is called the "companion layer". The hair bulb is the basal part of the hair follicle, and is composed of special tissues including dermal papilla, hair matrix, and precursor cells of the internal root sheath. Development of the hair follicle implies the coordinated differentiation of these tissues. During differentiation of tissues of the internal root sheath and hair shaft, most cellular organelles disappear and are replaced with keratin fibers. We conducted electron microscopic and immunohistochemical studies in hair follicles of the dorsal skin of neonate rats to investigate the roles of autophagy and proteolysis in this conversion. Autophagy was frequently found in the hair shaft during the keratinization process, especially in the hair cuticle area. Immunohistochemical study revealed the striking co-localization of cathepsin B, cathepsin D, cathepsin L, LAMP-1 and μ-calpain in the external root sheath, keratinizing Henle's layer, keratinizing hair shaft and primitive sebaceous gland suggesting the coordination of lysosomal proteases with cytoplasmic exzymes in promotion of the cytoplasmic conversion or terminal differentiation of hair follicle cells.

• 日本組織細胞化学会の論文

著者

• Kominami Eiki

  Department of Biochemistry, Juntendo University School of Medicine

• SAKURABA HITOSHI

  Department of Clinical Genetics, the Tokyo Metropolitan Institute of Medical Science, Tokyo Metropol

• UENO Takashi

  Department of Dermatology, Nippon Medical School

• Sakuraba H

  Department Of Analytical Biochemistry Meiji Pharmaceutical University

• Ueno Takashi

  Department Of Biochemistry Juntendo University School Of Medicine

• Ueno T

  Toxicological Research Center Nitto Denko Corporation

• Kominami E

  Department Of Biochemistry Juntendo University School Of Medicine

• Kominami Eiki

  Dep. Of Biochemistry Juntendo Univ. School Of Medicine

• Kominami Eiki

  Department Of Biochemistry Faculty Of Medicine Juntendo University

• KAWASHIMA Seiichi

  Department of Molecular Biology, Tokyo Metropolitan Institute of Medical Science

• Kawashima Seiichi

  Dept. Of Mol. Biol. The Tokyo Metropolitan Inst. Med. Sci

• Kawashima Seiichi

  Department Of Biochemistry Tokyo Metropolitan Institute Of Gerontology

• Sato(kusubata) Kaori

  Dept. Of Mol. Biol. The Tokyo Metropolitan Inst. Med. Sci

• Ueno Takashi

  Dept. of Biochemistry, Juntendo University, School of medicine

• Sakuraba HItoshi

  Dept. of Clin. Genet., The Tokyo Metropolitan Inst. Med. Sci

• Ihara Setsunosuke

  Dept. of Biol. ScL, Shimane University, School of Life and Environmental Science

• Morioka Kiyokazu

  Department of Clinical Genetics, The Tokyo Metropolitan Institute of Medical Science

• Sato-Kusubata Kaori

  Department of Molecular Biology, The Tokyo Metropolitan Institute of Medical Science

• Ihara Setsunosuke

  Department of Biological Science, Faculty of Life and Environmental Science, Shimane University

• Sakuraba Hitoshi

  Department Of Analytical Biochemistry Meiji Pharmaceutical University

• Sakuraba Hitoshi

  Dept. Of Clin. Genet. The Tokyo Metropolitan Inst. Med. Sci

• Morioka Kiyokazu

  Dept. Of Clin. Genet. The Tokyo Metropolitan Inst. Med. Sci

• Morioka Kiyokazu

  Department Of Clinical Genetics The Tokyo Metropolitan Institute Of Medical Science

• Ihara Setsunosuke

  Dept. Of Biol. Scl Shimane University School Of Life And Environmental Science

• Ihara Setsunosuke

  Department Of Biological Science Faculty Of Life And Environmental Science Shimane University

• Morioka K

  The Tokyo Metropolitan Institute Of Medical Science

• Ueno T

  Toshiba Corp. Kawasaki‐shi Jpn

• Ueno Takashi

  Dept. Of Biochemistry Juntendo University School Of Medicine

• Ueno Takashi

  Department Of Bio-medical Engineering Tokai University

• Sakuraba H

  Department Of Tumor Immunology And Clinical Genetics Tokyo Metropolitan Institute Of Medical Science

• Ueno Takashi

  Department Of Applied Chemistry Faculty Of Science And Engineering Ritsumeikan University

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