Analysis of Where and Which Types of Proteinases Participate in Lysosomal Proteinase Processing Using Bafilomycin A1 and Helicobacter pylori Vac A Toxin.
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概要
- 論文の詳細を見る
Lysosomal proteinases are translated as preproforms, transported through the Golgi apparatus as proforms, and localized in lysosomes as mature forms. In this study, we analyzed which subclass of proteinases participates in the processing of lysosomal proteinases using Bafilomycin A 1, a vacuolar ATPase inhibitor. Bafilomycin A 1 raises lysosomal pH resulting in the degradation of lysosomal proteinases such as cathepsins B, D, and L. Twenty-four hours after the withdrawal of Bafilomycin A 1, NIH3T3 cells possess these proteinases in amounts and activities similar to those in cells cultured in DMEM and 5% BCS. In the presence of various proteinase inhibitors, procathepsin processing is disturbed by E-64-d, resulting in abnormal processing of cathepsins D and L, but not by APMSF, Pepstatin A, or CA-074. In the presence of Helicobacter pylori Vac A toxin, which prevents vesicular transport from late endosomes to lysosomes, the processing of procathepsins B and D occurs, while that of procathepsin L does not. Thus, procathepsins B and D are converted to their mature forms in late endosomes, while procathepsin L is processed to the mature form after its arrival in lysosomes by some cysteine proteinase other than cathepsin B.
- 社団法人 日本生化学会の論文
著者
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Aihara Miki
Microbiological Research Institute Otsuka Pharmaceutical Co. Ltd.
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Kominami Eiki
Department Of Biochemistry Faculty Of Medicine Juntendo University
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Watanabe Sumio
Department Of Gastroenterology Juntendo University
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Imagawa Kenichi
Microbiological Research Institute Otsuka Pharmaceutical Co Ltd
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Ishidoh Kazumi
Department Of Biochemistry Juntendo University School Of Medicine
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Kikuchi Mikio
Microbiological Research Institute Otsuka Pharmaceutical Co. Ltd.
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Takeda-ezaki Mitsue
Department Of Biochemistry Juntendo University School Of Medicine
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Sato Nobuhiro
Department of Cardio-angiology, Kitasato University School of Medicine
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