The Reconstituted Type I Collagen Fibrils from Skins of Variously Aged Bovines Exert Different Effects on the Morphology and Integrin α2β1 Expression of Fibroblasts in Culture
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概要
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We examined the effect of type I collagen extracted from the variously aged bovines when used as the substrate for skin fibroblast culture. We could not find any apparent difference in the cell adhesion and the expression of integrin α2β1 in the fibroblasts cultured on the non-fibrous form of type I collagens from the variously aged animals. But some prominent differences were observed between the collagens from young and old animals when the cells were cultured on the reconstituted type I collagen fibril. The morphology of reconstituted collagen fibrils was different depending on the animal age. The collagen isolated from young bovine skin assembled into thick fibrils, Whereas the collagen from aged bovine assembled into thin fibrils. The fibroblasts cultured on type I collagen fibrils adhered on fibril surface from all aged animals. But they spread well only on the fibrils from young bovine collagen whereas the cell spreading was not observed on the fibril from 8-year bovine collagen within 1 h. Gel contraction was examined by the in-gel culture. No gel contraction occurred in the collagens from 18 month-and 8 year-old bovines, while the contraction of the gel occurred in the collagens from bovines younger than 3-month old. To investigate the molecular mechanism underlying these phenomena, the expression of the most responsible adhesion molecule, integrin α2β1, was examined by immunoblotting. The expression of integrin α2β1 in fibroblasts was higher in the collagen gels from young animals than that from aged animals. These results show that the collagens undergo different age-dependent molecular assembly and exert different effects on the features of cell adhesion.
- 日本結合組織学会の論文
- 2000-03-25
著者
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ADACHI Eijiro
Department of Anatomy and Cell Biology, Kitasato University School of Medicine
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Adachi E
Department Of Molecular Morphology Kitasato University Graduate School Of Medicine
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Adachi Eijiro
Department Of Anatomy And Cell Biology Kitasato University School Of Medicine
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Hattori S
Nippi Research Institute Of Biomatrix And Japan Institute Of Leather Research
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Hattori Shunji
Nippi Research Institute Of Biomatrix
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Ebihara Tetsuya
Nippi Research Institute of Biomatrix
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Irie Shinkichi
Nippi Research Institute of Biomatrix
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KAWASHIMA Seiichi
Department of Molecular Biology, Tokyo Metropolitan Institute of Medical Science
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Irie S
Nippi Research Institute Of Biomatrix
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Irie Shinkichi
Research Institute Of Biomatrix Nippi Inc.
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Kawashima S
Department Of Molecular Biology Tokyo Metropolitan Institute Of Medical Science
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Kawashima Seiichi
Department Of Molecular Biology Tokyo Metropolitan Institute Of Medical Science
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Kawashima Seiichi
Department Of Molecular Biology Tokyo Metropolitan Institute For Medical Science
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Kawashima Seiichi
Department Of Biochemistry Tokyo Metropolitan Institute Of Gerontology
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Sato Kaori
Department Of Molecular Biology Tokyo Metropolitan Institute Of Medical Science
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SATO Kaori
Nippi Research Institute of Biomatrix
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Hattori Shunji
Nippi Res. Inst. Of Biomatrix Jpn
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Ebihara Tetsuya
Nippi Research Institute Of Biomatrix Nippi Inc.
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Hattori Shunji
Nippi Research Institute Of Biomatrix And Institute Of Leather Research Nippi Co. Ltd.
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Irie S
Nippi Res. Inst. Biomatrix Tokyo Jpn
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