A Novel Enzymatic Method for Measuring Mizoribine 5'-Monophosphate Levels in Serum(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
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概要
- 論文の詳細を見る
Inosine 5'-monophosphate dehydrogenases (IMPDH) from Oceanobacillus iheyensis and Bacillus subtilis were biochemically characterized with the aim of establishing a mizoribine 5'-monophosphate (MZR-P) assay. MZR-P is the active metabolite of mizoribine, which has been successfully used as an immunosuppressive agent. A sensitive method for measuring the MZR-P concentration in serum, based on its ability to inhibit IMPDH, was developed. The method was applied to an automatic clinical analyzer, enabling measurement of MZR-P levels in a set of serum samples. MZR-P at concentrations of 0.1-13.6 (μg/ml (0.3-40μM) in the samples could be measured with 0.7% within-run coefficients of variation.
- 社団法人日本生物工学会の論文
- 2008-11-25
著者
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Tamura Tomohiro
Research Institute of Genome-based Biofactory, AIST
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Tamura Tomohiro
Res. Inst. Of Genome-based Biofactory Aist Toyohira-ku Sapporo 062-8517 Jpn
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Tamura Tomohiro
Research Institute Of Genome-based Biofactory Aist:laboratory Of Molecular Environmental Microbiolog
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Yasuda Yuko
Asahi Kasei Pharma Corporation
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SAKASEGAWA Shin-ichi
Asahi Kasei Corporation
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Ota Hiroko
Asahi Kasei Pharma Corporation
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Imamura Shigeyuki
Asahi Kasei Pharma Corporation
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Yasuda Yuko
Asahi Kasei Pharma Corp. Shizuoka
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Sakasegawa Shin-ichi
Asahi Kasei Pharma Corporation
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Tamura Tomohiro
Research Institute Of Genome-based Biofactory National Institute Of Advanced Industrial Science And
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