Phylogenetic analysis of Rhodococcus erythropolis based on the variation of ribosomal proteins as observed by matrix-assisted laser desorption ionization-mass spectrometry without using genome information(METHODS)
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概要
- 論文の詳細を見る
Rhodococcus erythropolis strains characterized as antibiotic producers can be classified into three groups according to their antibiotic spectrum and growth compatibility. Due to their high genotypic similarity, the taxonomic relationship of these strains has not been elucidated. In this study, ribosomal protein profiling using matrix-assisted laser desorption ionization-mass spectrometry (MALDI-MS) was employed to classify twenty-one strains of R. erythropolis (15 antibiotic producers and 6 non-antibiotic producers). In the first step in this method, a total of 30 intense peaks observed for purified ribosomal subunit proteins of the type strain (R. erythropolis JCM 3201^T) were selected as the reference peaks. The mass spectra observed for the cell lysates of each sample strain were then checked as to whether peaks were observed at the same masses of the reference peaks. The results of peak matching were processed by cluster analysis, generating a dendrogram. Four major clusters of the R. erythropolis strains corresponded to three antibiotic groups and the non-antibiotic group. Furthermore, the topology of the dendrogram was highly comparable with the phylogenetic tree based on DNA gyrase subunit B gene (gyrB) sequencing. These results indicate that our proposed ribosomal protein profiling method using MALDI-MS is a potentially reliable and sufficiently high-throughput technique for the taxonomic analysis of closely related bacterial strains without using DNA sequence information.
- 社団法人日本生物工学会の論文
著者
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Tao Hiroaki
Research Institute For Environmental Management Technology National Institute Of Advanced Industrial
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Teramoto Kanae
Research Institute for Environmental Management Technology, National Institute of Advanced Industria
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Kitagawa Wataru
Research Institute of Genome-based Biofactory, AIST
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Sato Hiroaki
Research Institute for Environmental Management Technology, National Institute of Advanced Industria
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Torimura Masaki
Research Institute for Environmental Management Technology, National Institute of Advanced Industria
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Tamura Tomohiro
Research Institute of Genome-based Biofactory, AIST
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Tao H
National Inst. Of Advanced Industrial Sci. And Technol. (aist)
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Teramoto Kanae
Research Institute For Environmental Management Technology National Institute Of Advanced Industrial
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Tamura Tomohiro
Research Institute Of Genome-based Biofactory Aist:laboratory Of Molecular Environmental Microbiolog
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Kitagawa Wataru
Research Institute Of Genome-based Biofactory Aist
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Torimura Masaki
Research Institute For Environmental Management Technology National Institute Of Advanced Industrial
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Sato Hiroaki
Research Institute For Environmental Management Technology National Institute Of Advanced Industrial
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Torimura Masaki
Research Institute For Environmental Management Technology National Institute Of Advanced Industrial
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