A Novel Proteinaceous Kex 2 Proteinase Inhibitor, Kexstatin, from Streptomyces platensis Q268

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概要

• 論文の詳細を見る

• We found a novel proteinaceous Kex 2 proteinase inhibitor, named kexstatin, in the culture supernatant of Streptomyces platensis Q268.The purified kexstatin was homogeneous by SDS-PAGE and the molecular weight was estimated to be 13,000. The N-terminal amino acid sequence of kexstatin has high similarity to Streptomyces subtilisin inhibitor (SSI), suggesting that kexstatin belongs to the SSI family. Kexstatin was a strong inhibitor of Kex 2 proteinase and subtilisin but not thermolysin, trypsin, or chymotrypsin. The IC_<50> value of kexstatin against 1 μg of Kex 2 proteinase was 1.4 μg.

• 社団法人日本農芸化学会の論文
• 1996-08-23

著者

• ODA Kohei

  Department of Applied Biology, Kyoto Institute of Technology

• Takahashi S

  Akita Res. Inst. Food And Brewing Akita

• TAKAHASHI Saori

  Department of Bioengineering, Akita Research Institute of Food and Brewing (ARIF)

• Oda Kohei

  Department Of Agricultural Chemistry College Of Agriculture University Of Osaka Prefecture

• KIKUCHI Norihisa

  Biomolecular Engineering Research Institute

• SHIBANO Yuji

  Biomolecular Engineering Research Institute

• Shibano Yuji

  Suntory Research Center:(present Address)suntory Ltd.

• Shibano Yuji

  Department Of Molecular Biology Biomolecular Engineering Research Institute

• Shibano Yuji

  Department Of Moleular Biology Biomolecular Engineering Research Insitute

• Oda K

  Department Of Applied Biology Faculty Of Textile Science Kyoto Institute Of Technology

• Kikuchi N

  Shionogi Hyogo Jpn

• Suzuki Takahito

  Biological Laboratory Faculty Of Science Nara Women's University

• Takahashi Saori

  Department Of Applied Biology Faculty Of Textile Science Kyoto Institute Of Technology

• Yamashita Satoshi

  Department Of Biochemistry And Engineering Graduate School Of Engineering Tohoku University

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