Isolation and Some Properties of a Novel Killer Toxin-like Protein Produced by Streptomyces sp. F-287
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概要
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A killer toxin-like protein was found in the culture supernatant of a strain isolated from soil. The strain was classified and designated as Streptomyces sp. F-287. The molecular weight of the purified killer toxin-like protein was estimated to be 9,500 by SDS-PAGE. The purified protein was heat stable (100℃, 5 min), pH stable (pH 6.0-9.0,60℃, for 30 min), and had a relatively wide action spectra. The SKLP showed a cytocidal effect on both budding yeast, Saccharomyces cerevisiae W303 (IC_50=15.6μg/ml) and on fission yeast, Schizosaccharomyces pombe SP870 (IC_50=20.0μg/ml). The SKLP also caused morphological changes on some sensitive yeasts and filamentous fungi. These characteristics are apparently different from known killer toxins. These results suggest that this is a novel killer toxin-like protein from Streptomyces sp. strain F-287.
- 社団法人日本農芸化学会の論文
- 1999-06-23
著者
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Hiraga Kazumi
Department of Applied Biology, Kyoto Institute of Technology
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ODA Kohei
Department of Applied Biology, Kyoto Institute of Technology
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Hiraga K
Kyoto Inst. Technol. Kyoto Jpn
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Hiraga Kazumi
Department Of Applied Biology Faculty Of Textile Science Kyoto Institute Of Technology
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Hiraga K
Department Of Applied Biology Faculty Of Textile Science Kyoto Institute Of Technology
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HAYASHI Suzuko
Department of Applied Biology, Faculty of Textile Science, Kyoto Institute of Technology
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KITAZAWA Mitsunori
Department of Applied Biology, Faculty of Textile Science, Kyoto Institute of Technology
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Oda Kohei
Department Of Agricultural Chemistry College Of Agriculture University Of Osaka Prefecture
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Kitazawa M
Department Of Applied Biology Faculty Of Textile Science Kyoto Institute Of Technology
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Kitazawa Mitsunori
Department Of Applied Biology Faculty Of Textile Science Kyoto Institute Of Technology
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Hayashi Suzuko
Department Of Applied Biology Faculty Of Textile Science Kyoto Institute Of Technology
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Oda K
Department Of Applied Biology Faculty Of Textile Science Kyoto Institute Of Technology
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