Inactivation of Scytatidium lignicolum Acid Protease B with 1,2-Epoxy-3-(4'-azido-2'-nitrophenoxy)propanet(Biological Chemistry)
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概要
- 論文の詳細を見る
The acid protease B of Scytalidium lignicolum (SAB) was modified with 1,2-epoxy-3-(4'-azido-2'-nitrophenoxy)propane [EANP] under acidic conditions. EANP was incorporated stoichiometrically into SAB, and the enzyme was almost completely inactivated. The modified enzyme was digested with thermolysin and EANP-labeled peptides were separated by high performance liquid chromatography (HPLC). The amino acid sequence of the major peptide labeled was Ile-Leu-Glu-Thr-Gly, which corresponds to the sequence of residue Nos. 51〜55 of the enzyme. Treatment of the peptide with 0.5 N hydroxylamine (pH 10.5) resulted in the complete removal of the modifier from the labeled peptide, suggesting that EANP is ester-linked to the Glu-53 residue of the enzyme. The amino acid sequence (-Cys-Gln-Thr-Ala-Ile-Leu-Glu-Thr-Gly-) around Glu-53 of SAB shows high homology with those around the active site aspartic acids of calf chymosin and porcine pepsin. The results indicate the involvement of Glu-53 in the enzyme reaction of SAB.
- 社団法人日本農芸化学会の論文
- 1989-10-23
著者
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YOSHIMOTO TADASHI
School of Pharmaceutical Sciences, Nagasaki University
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TSURU DAISUKE
School of Pharmaceutical Sciences, Nagasaki University
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Yoshimoto T
Nagasaki Univ. Nagasaki Jpn
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Yoshlmoto Tadashi
School Of Pharmaceutical Sciences Nagasaki University
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Yoshimoto Tadashi
School Of Pharmaceutical Sciences Nagasaki University
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Yoshimoto Tadashi
School Of Pharmaceutical Sciences Nagasaki University:yakult Central Institute For Microbiological R
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Yoshimoto Tadashi
School Of Pharmaceutical Sciences Nagasiki University
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Murao S
Department Of Applied Biology Faculty Of Textile Science Kyoto Institute Of Technology
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KOBAYASHI Ryuji
School of Pharmaceutical Sciences, Nagasaki University
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Kobayashi Ryuji
School Of Pharmaceutical Sciences Nagasaki University
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Kobayashi Ryuji
School Of Health Science Kibi International University
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Murao S
Sojo Univ. Kumamoto Jpn
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Tsuru D
School Of Pharmaceutical Sciences Nagasaki University:yakult Central Institute For Microbiological R
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Tsuru Daisuke
Department Of Applied Microbial Technology Sojo University
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Oda K
Department Of Applied Biology Faculty Of Textile Science Kyoto Institute Of Technology
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Tsuru D
Department Of Applied Microbial Technology Sojo University
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NAOTSUKA Akihiko
School of Pharmaceutical Sciences, Nagasaki University
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ODA Kohei
University of Osaka Prefecture
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MURAO Sawao
Kumamoto Institute of Technology
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Tsuru Daisuke
School Of Pharmaceutical Sciences Nagasaki University
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Yoshimoto Tomotaka
Department Of Forest Products Faculty Of Agriculture The University Of Tokyo
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Naotsuka Akihiko
School Of Pharmaceutical Sciences Nagasaki University
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Yoshimoto Tadashi
School of Pharamaceutical Sciences, Nagasaki University
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