Nucleotide Sequence of the Neutral Protease Gene from Bacillus subtilis var. amylosacchariticus

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概要

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• The neutral protease gene of Bacillus subtilis var. amylosacchariticus was isolated and the nucleotide sequence encoding the signal and pro-peptides followed by the mature protein region was established. Putative -35 and -10 sequences, TTGAGT and TAATAT, were observed as the consensus sequence for the promoter recognized by the σ^<55> RNA polymerase of B. subtilis, and the ribosome binding site, whose sequence was AAAGGGGG, was complementary to the binding sequence of B. subtilis 16s rRNA except for one base. The nucleotide sequence of the gene was highly homologous to that of the neutral protease gene from B. subtilis IA72,particularly in the mature protein coding region : only two nucleotide discrepancies out of 900 nucleotides were found, which resulted in no amino acid replacement. However, sequences in pre-pro and putative terminator regions were significantly different between the neutral protease genes of these two strains of B. subtilis. In B. subtilis var. amylosacchariticus, a stem-loop structure with a calculated free energy of -46.0 kcal/mol, which was much lower than that of B. subtilis IA72,was postulated at the 3' terminal region.

• 社団法人日本生物工学会の論文
• 1990-12-25

著者

• Oyama H

  Kyoto Inst. Technol. Kyoto Jpn

• Xu Sheng-lin

  School Of Pharmaceutical Sciences Nagasaki University

• YOSHIMOTO TADASHI

  School of Pharmaceutical Sciences, Nagasaki University

• OYAMA HIROSHI

  School of Pharmaceutical Sciences, Nagasaki University

• TAKESHITA TOMOAKI

  School of Pharmaceutical Sciences, Nagasaki University

• HIGASHI HIDEAKI

  School of Pharmaceutical Sciences, Nagasaki University

• TSURU DAISUKE

  School of Pharmaceutical Sciences, Nagasaki University

• Yoshimoto T

  Nagasaki Univ. Nagasaki Jpn

• Yoshlmoto Tadashi

  School Of Pharmaceutical Sciences Nagasaki University

• Yoshimoto Tadashi

  School Of Pharmaceutical Sciences Nagasaki University

• Yoshimoto Tadashi

  School Of Pharmaceutical Sciences Nagasaki University:yakult Central Institute For Microbiological R

• Yoshimoto Tadashi

  School Of Pharmaceutical Sciences Nagasiki University

• Oyama Hiroshi

  Department Of Applied Biology Faculty Of Textile Science Kyoto Institute Of Technology

• Tsuru D

  School Of Pharmaceutical Sciences Nagasaki University:yakult Central Institute For Microbiological R

• Tsuru Daisuke

  Department Of Applied Microbial Technology Sojo University

• Tsuru D

  Department Of Applied Microbial Technology Sojo University

• Tsuru Daisuke

  School Of Pharmaceutical Sciences Nagasaki University

• Takeshita T

  Kyushu Univ. Fukuoka Jpn

• Yoshimoto Tomotaka

  Department Of Forest Products Faculty Of Agriculture The University Of Tokyo

• Yoshimoto Tadashi

  School of Pharamaceutical Sciences, Nagasaki University

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