Cloning and Expression of the cDNA Encoding Prolyl Oligopeptidase (Prolyl Endopeptidase) from Bovine Brain

元データ 1997-10-15 公益社団法人日本薬学会

概要

Prolyl oligopeptidase (EC 3.4.21.26,prolyl endopeptidase) cDNA from bovine brain was cloned by PCR, and the amplified fragment was used as a probe to screen the cDNA library from bovine brain. The obtained clone contained a 2.7 kb DNA fragment with an open reading frame of 2130 nucleotides, and encoded a protein of 710 amino acids with a deduced molecular weight of 80640 Da. The deduced amino acid sequence is 95,94,51 and 48% homologous to those of human T-cell, porcine brain, Aeromonas hydrophila, and Flavobacterium meningosepticum prolyl endopeptidases, respectively. The bovine brain prolyl endopeptidase-encoding cDNA was expressed using an expression vector bearing a tac promoter, with an approximate yield of 20 μg/ml of cell culture.

著者

Kabashima T School Of Pharmaceutical Sciences Nagasaki University
Kabashima Tsutomu School Of Pharmaceutical Sciences Nagasaki University
吉田 豊和 岐阜大・工・生命工
Ito Kiyoshi School of Pharmaceutical Sciences, Nagasaki University
Ito K School Of Pharmaceutical Sciences Nagasaki University
YOSHIMOTO TADASHI School of Pharmaceutical Sciences, Nagasaki University
Yoshimoto Tadashi School Of Pharmaceutical Sciences Nagasaki University
Ito K Sapporo Breweries Ltd. Gumma Jpn
Ito Kiyoshi School Of Pharmaceutical Sciences Nagasaki University
MIYAZAKI Kaori School of Pharmaceutical Sciences, Nagasaki University
HARAGUCHI Naoko School of Pharmaceutical Sciences, Nagasaki University
KITAZONO Ana School of Pharmaceutical Sciences, Nagasaki University
Kitazono Ana School Of Pharmaceutical Sciences. Nagasaki University
Miyazaki Kaori School Of Pharmaceutical Sciences Nagasaki University
Haraguchi Naoko School Of Pharmaceutical Sciences Nagasaki University
Ito K Yamagata Univ. Yonezawa Jpn
Yoshimoto Tadashi School of Pharamaceutical Sciences, Nagasaki University

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