Cloning and Expression of the cDNA Encoding Prolyl Oligopeptidase (Prolyl Endopeptidase) from Bovine Brain
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概要
- 論文の詳細を見る
Prolyl oligopeptidase (EC 3.4.21.26,prolyl endopeptidase) cDNA from bovine brain was cloned by PCR, and the amplified fragment was used as a probe to screen the cDNA library from bovine brain. The obtained clone contained a 2.7 kb DNA fragment with an open reading frame of 2130 nucleotides, and encoded a protein of 710 amino acids with a deduced molecular weight of 80640 Da. The deduced amino acid sequence is 95,94,51 and 48% homologous to those of human T-cell, porcine brain, Aeromonas hydrophila, and Flavobacterium meningosepticum prolyl endopeptidases, respectively. The bovine brain prolyl endopeptidase-encoding cDNA was expressed using an expression vector bearing a tac promoter, with an approximate yield of 20 μg/ml of cell culture.
- 公益社団法人日本薬学会の論文
- 1997-10-15
著者
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Kabashima T
School Of Pharmaceutical Sciences Nagasaki University
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Kabashima Tsutomu
School Of Pharmaceutical Sciences Nagasaki University
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吉田 豊和
岐阜大・工・生命工
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Ito Kiyoshi
School of Pharmaceutical Sciences, Nagasaki University
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Ito K
School Of Pharmaceutical Sciences Nagasaki University
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YOSHIMOTO TADASHI
School of Pharmaceutical Sciences, Nagasaki University
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Yoshimoto Tadashi
School Of Pharmaceutical Sciences Nagasaki University
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Ito K
Sapporo Breweries Ltd. Gumma Jpn
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Ito Kiyoshi
School Of Pharmaceutical Sciences Nagasaki University
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MIYAZAKI Kaori
School of Pharmaceutical Sciences, Nagasaki University
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HARAGUCHI Naoko
School of Pharmaceutical Sciences, Nagasaki University
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KITAZONO Ana
School of Pharmaceutical Sciences, Nagasaki University
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Kitazono Ana
School Of Pharmaceutical Sciences. Nagasaki University
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Miyazaki Kaori
School Of Pharmaceutical Sciences Nagasaki University
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Haraguchi Naoko
School Of Pharmaceutical Sciences Nagasaki University
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Ito K
Yamagata Univ. Yonezawa Jpn
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Yoshimoto Tadashi
School of Pharamaceutical Sciences, Nagasaki University
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