Glutathione-independent Formaldehyde Dehydrogenase from Pseudomons putida : Survey of Functional Groups with Special Regard for Cysteine Residues
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概要
- 論文の詳細を見る
The role of cysteine residues for structure and function of formaldehyde dehydrogenase from Pseudomonas putida was analysed by amino acid sequence comparison, homology-based structure modeling, site-directed mutagenesis, and chemical modification. Five out of seven cysteine residues found in the enzyme were concluded to coordinate with an active site zinc (Cys-46) and structural zinc atoms (Cys-97, -100, -103, and -111) from the sequence comparison with other Zn-containing medium-chain alcohol dehydrogenase homologues. The three-dimensional structure model based on the known structure of the horse liver E-type alcohol dehydrogenase (ADH) indicated that Cys-257 is located very far from the active site Zn and NAD^+ binding region, suggesting that Cys-257 does not participate in the enzyme reaction. The structure also suggested that Cys-166 does not coordinate to active site Zn, but Asp-169 functions as a Zn-ligand, instead.
- 社団法人日本農芸化学会の論文
- 1997-08-23
著者
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Ito Kiyoshi
School of Pharmaceutical Sciences, Nagasaki University
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Tsuru Daisuke
Department of Applied Microbiology, Kumamoto Institute of Technology
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Ito K
School Of Pharmaceutical Sciences Nagasaki University
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YOSHIMOTO TADASHI
School of Pharmaceutical Sciences, Nagasaki University
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Yoshimoto T
Nagasaki Univ. Nagasaki Jpn
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Yoshlmoto Tadashi
School Of Pharmaceutical Sciences Nagasaki University
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Yoshimoto Tadashi
School Of Pharmaceutical Sciences Nagasaki University
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Yoshimoto Tadashi
School Of Pharmaceutical Sciences Nagasaki University:yakult Central Institute For Microbiological R
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Yoshimoto Tadashi
School Of Pharmaceutical Sciences Nagasiki University
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Ito K
Sapporo Breweries Ltd. Gumma Jpn
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ODA Naoko
Department of Applied Microbial Technology, Kumamoto Institute of Technology
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MATSUO Yo
Institute for Social Information Science, Fujitsu Laboratories Ltd.
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ISHIKAWA Sara
School of Pharamaceutical Sciences, Nagasaki University
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Ito Kiyoshi
School Of Pharmaceutical Sciences Nagasaki University
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Oda Naoko
Department Of Applied Microbial Technology Kumamoto Institute Of Technology
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Matsuo Y
Department Of Applied Bioscience And Biotechnology Faculty Of Life And Environmental Science Shimane
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Ishikawa Sara
School Of Pharamaceutical Sciences Nagasaki University
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Tsuru D
School Of Pharmaceutical Sciences Nagasaki University:yakult Central Institute For Microbiological R
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Tsuru Daisuke
Department Of Applied Microbial Technology Sojo University
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Tsuru Daisuke
Department Of Applied Microbial Technology Kumamoto Institute Of Technology
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Tsuru D
Department Of Applied Microbial Technology Sojo University
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Yoshimoto Tomotaka
Department Of Forest Products Faculty Of Agriculture The University Of Tokyo
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Ito K
Yamagata Univ. Yonezawa Jpn
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Yoshimoto Tadashi
School of Pharamaceutical Sciences, Nagasaki University
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