Purification and Characterization of Proline Iminopeptidase from Lyophyllum cinerascens

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概要

• 論文の詳細を見る

• Proline iminopeptidase (EC 3.4.11.5) was purified from an extract of Lyophyllum cinerascens by ammonium sulfate fractionation and sequential chromatographies on DEAE-Sephadex, Sephadex G-150,HPLC-Phenyl-5PW, and HPLC-DEAE-5PW column, with an activity recovery of 6.4%. The enzyme was a dimeric protein of molecular weight, 150,000. It exhibited pH optimum of 7.2 and stability between pH 5.2 and 8.8. The isoelectric point of the enzyme was 4.3. The enzyme catalyzed the hydrolysis of Pro-X (X=amino acid, peptide and arylamide) bonds when the proline residue was the amino terminus. The enzyme was resistant to the action of PCMB. Neither sulfhydryl reagents nor serine protease inhibitors affected the enzyme activity. The enzyme was not inhibited by EDTA and o-phenantheroline. It was, however, strongly inhibited by prolinol and diethyl pyrocarbonate. The enzyme showed a sharp precipitin line on immunodiffusion against its antiserum developed in rabbit.

• 社団法人日本生物工学会の論文
• 1989-09-25

著者

• YOSHIMOTO TADASHI

  School of Pharmaceutical Sciences, Nagasaki University

• TSURU DAISUKE

  School of Pharmaceutical Sciences, Nagasaki University

• Yoshimoto T

  Nagasaki Univ. Nagasaki Jpn

• Yoshlmoto Tadashi

  School Of Pharmaceutical Sciences Nagasaki University

• Yoshimoto Tadashi

  School Of Pharmaceutical Sciences Nagasaki University

• Yoshimoto Tadashi

  School Of Pharmaceutical Sciences Nagasaki University:yakult Central Institute For Microbiological R

• Yoshimoto Tadashi

  School Of Pharmaceutical Sciences Nagasiki University

• Tsuru D

  School Of Pharmaceutical Sciences Nagasaki University:yakult Central Institute For Microbiological R

• Tsuru Daisuke

  Department Of Applied Microbial Technology Sojo University

• Tsuru D

  Department Of Applied Microbial Technology Sojo University

• Tsuru Daisuke

  School Of Pharmaceutical Sciences Nagasaki University

• Yoshimoto Tomotaka

  Department Of Forest Products Faculty Of Agriculture The University Of Tokyo

• Yoshimoto Tadashi

  School of Pharamaceutical Sciences, Nagasaki University

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