Cloning of L-Amino Acid Deaminase Gene from Proteus vulgaris
スポンサーリンク
概要
- 論文の詳細を見る
The L-amino acid degrading enzyme gene from Proteus vulgaris was cloned and the nucleotide sequence of the enzyme gene was clarified. An open reading frame of 1,413 bp starting at an ATG methionine codon was found, which encodes a protein of 471 amino acid residues, the calculated molecular weight of which is 51,518. The amino acid sequence of P. vulgaris was 58.6% identical it the L-amino acid deaminase of P. mirabilis. A significantly conserved sequence was found around the FAD-binding sequence of flavo-proteins. The partially purified wild and recombinant enzymes had the same substrate specificity for L-amino acids to form the respective keto-acids, however not for D-amino acids.
- 社団法人日本農芸化学会の論文
- 1999-12-23
著者
-
YOSHIMOTO TADASHI
School of Pharmaceutical Sciences, Nagasaki University
-
Yoshimoto Tadashi
School Of Pharmaceutical Sciences Nagasaki University
-
Ito Kiyoshi
School Of Pharmaceutical Sciences Nagasaki University
-
Takahashi Eiji
Basic Technology Department Discovery Research Laboratory Tanabe Seiyaku Co. Ltd.
-
Yoshimoto Tadashi
School of Pharamaceutical Sciences, Nagasaki University
関連論文
- 2A16-4 epsilon-Poly-L-lysine(PL)分解酵素遺伝子の取得と破壊株を用いた機能解析(遺伝子工学・核酸工学,一般講演)
- 3P-1072 エナンチオ選択的イミン還元酵素の探索と特徴解析(2a酵素学,酵素工学,一般講演,酵素学,タンパク質工学および酵素工学,伝統の技と先端科学技術の融合)
- Prolidase from Xanthomonas maltophilia : Purification and Characterization of the Enzyme
- Nucleotide Sequence of the Neutral Protease Gene from Bacillus subtilis var. amylosacchariticus
- Glutathione-independent Formaldehyde Dehydrogenase from Pseudomons putida : Survey of Functional Groups with Special Regard for Cysteine Residues
- Prolylcarboxypeptidase (Angiotensinase C) : Purification and Characterization of the Enzyme from Xanthomonas maltophilia
- Degradation of Streptomyces Metalloprotease Inhibitor (SMPI) by Neutral Protease from Bacillus subtilis var. amylosacchariticus
- CPT-11 Converting Enzyme from Rat Serum : Purification and Some Properties
- NOVEL AND POTENT INHIBITORS SPECIFIC FOR PROLYL ENDOPEPTIDASE AND THEIR ANTI-AMNESIC EFFECT
- SPECIFIC INHIBITORS FOR PROLYL ENDOPEPTIDASE AND THEIR ANTI-AMNESIC EFFECT
- Specific Inhibition of Dipeptidyl Aminopeptidase IV by a New Synthetic Inhibitor, L-Thioprolylthiazolidine(Biological Chemistry)
- Structure Activity Relationship of Inhibitors Specific for Prolyl Endopeptidase(Biological Chemistry)
- Purification and Characterization of Proline Iminopeptidase from Lyophyllum cinerascens
- 516 Studies on Prolyl Endopeptidase from Shakashimeji (Lyophyllum Cinerascens) : Purification and Enzymatic Properties.
- Effects of an 'enrichment feeder' on behavior and feather conditions of caged laying hens
- 2Fp04 シアノヒドリンに作用するニトリラーゼの特性(酵素学,酵素工学,タンパク質工学,一般講演)
- 2Fp03 微生物による1,3-アダマンタンジオールの位置選択的水酸化(酵素学,酵素工学,タンパク質工学,一般講演)
- 2Fp05 Fusarium属カビのニトリラーゼの分子特性の解析(酵素学,酵素工学,タンパク質工学,一般講演)
- 1Fp09 4-ヒドロキシ安息香酸脱炭酸酵素の構造と機能発現の相関(酵素学,酵素工学,タンパク質工学,一般講演)
- 2B15-2 マンデロニトリルに作用する立体選択的ニトリラーゼ(酵素学・酵素工学・タンパク質工学,一般講演)
- 1F15-2 イソオイゲノール分解酵素の遺伝子クローニングと一次構造解析(酵素学,酵素工学,タンパク質工学,一般講演)
- 1F15-3 二相系におけるイソオイゲノールのバニリンへの微生物変換(酵素学,酵素工学,タンパク質工学,一般講演)
- 可逆的脱炭酸酵素の特性解析と芳香族カルボン酸の合成について
- ACTIVE SITE STRUCTURE AND FUNCTION OF NEUTRAL PROTEASE FROM BACILLUS SUBTILIS var. AMYLOSACCHARITICUS
- Chemical Modification of Neutral Protease from Bacillus subtilis var. amylosacchariticus : Assignment of Tyrosyl Residues Iodinated(Biological Chemistry)
- Dye-sensitized Photooxidation of Neutral Protease from Bacillus subtilis var. amylosacchariticus : Assignment of Histidine Residue Oxidized(Biological Chemistry)
- Nucleotide Sequence of the Gene Encoding Pepstatin-insensitive Acid Protease B, Scytalidopepsin B, of Scytalidium lignicolum
- Purification and Characterization of a Prolyl Endopeptidase from Pseudomonas sp. KU-22
- Substrate Recognition Mechanism of Proly Aminopeptidase from Serratia marcescens^1
- Glutamate 83 Is Important for Stabilization of Domain-Domain Conformation of Thermus aquaticus Glycerol Kinase
- Crystal Structure of Prolyl Aminopeptidase from Serratia marcescens
- Cloning, Sequencing, High Expression, and Crystallization of the Thermophile Thermus aquaticus Glycerol Kinase
- Roles of the Ser146, Tyr159, and Lys163 Residues in the Catalytic Action of 7α-Hydroxysteroid Dehydrogenase from Escherichia coli
- Cloning and Expression of the cDNA Encoding Prolyl Oligopeptidase (Prolyl Endopeptidase) from Bovine Brain
- Prolyl Aminopeptidase from Serratia marcescens: Cloning of the Enzyme Gene and Crystalliztion of the Expressed Enzyme^1
- Purification and Characterization of Thermostable Glycerol Kinase from Thermus flavus
- Dipeptidyl Peptidase IV from Xanthomonas maltophilia: Sequencing and Expression of the Enzyme Gene and Charactrization of the Expressed Enzyme
- Prolyl Aminopeptidase Is Also Present in Enterobacteriaceae: Cloning and Sequencing of the Hafnia alvei Enzyme-Gene and Characterization of the Expressed Enzyme
- Protease II from Moraxella lacunata: Cloning, Sequencing, and Expression of the Enzyme Gene, and Crystallization of the Expressed Enzyme
- Novel Aminopeptidase Specific for Glycine from Actinomucor elegans(Biochemistry & Molecular Biology)
- Volatile Compounds Produced by the Fungus Aspergillus oryzae in Rice Koji and Their Changes during Cultivation
- Mycelia Distribution in Rice Koji
- Effect of Processing on the Pore Size Distribution and Digestibility of Rice Grain(Food & Nutrition)
- Retention of Aroma Components in Liquors with Cyclodextrins(Food & Nutrition)
- Prolyl Endopeptidase Inhibitors Derived from Actinomycetes
- Secretion of Escherichia coli Aminopeptidase P in Bacillus subtilis Using the Prepro-Structure Coding Region of Subtilisin Amylosacchariticus
- Novel Prolyl Tri/Tetra-Peptidyl Aminopeptidase from Streptomyces mobaraensis : Substrate Specificity and Enzyme Gene Cloning
- CLONING OF AMINOPEPTIDASES P AND N GENES AND CHARACTERIZATION OF EXPRESSED ENZYMES
- Inactivation of Scytatidium lignicolum Acid Protease B with 1,2-Epoxy-3-(4'-azido-2'-nitrophenoxy)propanet(Biological Chemistry)
- Complete Amino Acid Sequence of Neutral Protease from Bacillus subtilis var. amylosacchariticus(Biological Chemistry)
- Inhibition of Scytalidium lignicolum Acid Protease B by 1-Diazo-3-phenyl-2-propanone(Biological Chemistry)
- Directed Evolution to Improve the Thermostability of Prolyl Endopeptidase^1
- A Novel Assay Method for Aminopeptidase P and Partial Purification of Two Types of the Enzyme in Escherichia coli(Biological Chemistry)
- An Aminopeptidase N from Escherichia coli HB101: Purification and Demonstration that the Enzyme Possesses Arylamidase and Peptidase Activitiest(Biological Chemistry)
- 406 Comparison of Prolyl Endopeptidases Produced in Culture Medium and Fruit-body of Agaricus bisporus (Mushroom)
- 有機溶媒耐性酵素を活用したケミカル生産(疎水性ケミカルのバイオプロダクションを目指した基盤研究)
- Behavioral Responses of Japanese Wild Boars to the Person in Attendance during the Pre- and Post-farrowing Periods under Captive Conditions
- Behavioral Responses of Japanese Wild Boars to the Person in Attendance during the Pre- and Post-farrowing Periods under Captive Conditions
- Color Discrimination in Dogs
- Color Discrimination in Dogs
- Behavior of Japanese Wild Boars, Sus Scrofa leucomystax, during the First Week after Parturition in Farrowing Pens
- Behavior of Japanese Wild Boars, Sus Scrofa leucomystax, during the First Week after Parturition in Farrowing Pens
- Courtship Behavior of Japanese Wild Boars, Sus scrofa leucomystax, under Captive Conditions
- Courtship Behavior of Japanese Wild Boars, Sus scrofa leucomystax, under Captive Conditions
- Purification and Enzymatic Properties of an Endo-α-N-Acetyl-Galactosaminidase from Bacillus laterosporus SS-16
- Are Litter Mates Needed for Each Piglet to Keep its Exclusive Teat after the Establishment of a Teat Order?
- 2Da05 アミノ基転移酵素による光学活性アミンの合成(酵素学・酵素工学,一般講演)
- 微生物の有機溶媒耐性酵素とそのケミカル生産への活用
- Cloning of L-Amino Acid Deaminase Gene from Proteus vulgaris
- 2Da04 酢酸菌による1,3-プロパンジオール類からアクリル酸類への変換(酵素学・酵素工学,一般講演)
- Membrane Topology and Functional Analysis of Methylobacillus sp. 12S Genes epsF and epsG, Encoding Polysaccharide Chain-Length Determining Proteins
- 2Cp23 Rhodococcus rhodochrous J1ニトリラーゼによるマロノニトリル誘導体の立体選択的加水分解(酵素学,酵素工学,一般講演)
- 2Cp22 (S)-イミン還元酵素による光学活性アミン合成(酵素学,酵素工学,一般講演)
- Courtship Behavior of Japanese Wild Boars, Sus scrofa leucomystax, under Captive Conditions
- Color Discrimination in Dogs
- 2P-042 様々なイミンに作用する(R)-イミン還元酵素の特徴解析(酵素学,酵素工学,一般講演)
- 2P-041 1-アセチル-2-メチルピロリジンを立体選択的に加水分解するハイドロラーゼの特徴解析(酵素学,酵素工学,一般講演)
- 2P-039 1,4-ジアセチル-2-メチルピペラジンに作用する立体特異的ハイドロラーゼの探索(酵素学,酵素工学,一般講演)
- Roles of the Ser146, Tyr159, and Lys163 Residues in the Catalytic Action of 7.ALPHA.-Hydroxysteroid Dehydrogenase from Escherichia coli.
- Prolyl Aminopeptidase Is Also Present in Enterobacteriaceae: Cloning and Sequencing of the Hafnia alvei Enzyme-Gene and Characterization of the Expressed Enzyme.
- Substrate Recognition Mechanism of Prolyl Aminopeptidase from Serratia marcescens.
- Crystal Structure of Prolyl Aminopeptidase from Serratia marcescens.
- Prolyl Aminopeptidase from Serratia marcescens: Cloning of the Enzyme Gene and Crystallization of the Expressed Enzyme.
- Novel Prolyl Tri/Tetra-Peptidyl Aminopeptidase from Streptomyces mobaraensis: Substrate Specificity and Enzyme Gene Cloning