Nucleotide Sequence of the Gene Encoding the Precursor Protein of Pepstatin insensitive Acid Protease B, Scytalidopepsin B, from Scytalidium lignicolum
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概要
- 論文の詳細を見る
A chromosomal DNA of Scytalidium lignicolum was digested with Sau3AI. The digest was self-ligated and amplified by inverse PCR procedure using primers designed based on the nucleotide sequences of up-and down-stream regions of an intron present in the scytalidopepsin B gene. Analysis of the nucleotide sequence of PCR product (700 bp) showed that the enzyme is synthesized as a precursor protein consisting of the prepro-and mature enzyme regions. The deduced amino acid sequence was highly similar to those of aspergillopepsin A and recently reported endothiapepsins B and C, but quite different from those of pepstatin-insensitive bacterial acid proteases and the pepstatin-sensitive aspartic protease family.
- 社団法人日本農芸化学会の論文
- 1998-08-23
著者
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ODA Kohei
Faculty of Science and Technology, Sophia University
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Oyama H
Kyoto Inst. Technol. Kyoto Jpn
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Gotoh Yoshikazu
Department of Cardiology, Kasumigaura Hospital, Tokyo Medical College
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Tsuru Daisuke
Department of Applied Microbiology, Kumamoto Institute of Technology
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ODA Naoko
Department of Applied Microbial Technology, Kumamoto Institute of Technology
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Murao Sawao
Department of Agricultural Chemistry, College of Agriculture, University of Osaka Prefecture
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Murao S
Department Of Applied Biology Faculty Of Textile Science Kyoto Institute Of Technology
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Murao S
Department Of Applied Biological Chemistry College Of Agriculture University Of Osaka Prefecture
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Morikawa Shin-ya
School Of Pharmaceutical Sciences Nagasaki University
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Oyama Hiroshi
Department Of Applied Biology Faculty Of Textile Science Kyoto Institute Of Technology
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Gotoh Yoshikazu
Department Of Applied Microbial Technology Kumamoto Institute Of Technology
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Oda Kohei
Faculty Of Science And Technology Sophia University
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Oda N
Department Of Applied Microbial Technology Sojo University
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Oda Naoko
Department Of Applied Microbial Technology Kumamoto Institute Of Technology
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Murao S
Sojo Univ. Kumamoto Jpn
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Murao Sawao
Department Of Agricultural Chemistry College Of Agriculture University Of Osaka Prefecture
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Tsuru D
School Of Pharmaceutical Sciences Nagasaki University:yakult Central Institute For Microbiological R
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Tsuru Daisuke
Department Of Applied Microbial Technology Sojo University
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Tsuru Daisuke
Department Of Applied Microbial Technology Kumamoto Institute Of Technology
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Oda K
Department Of Applied Biology Faculty Of Textile Science Kyoto Institute Of Technology
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Tsuru D
Department Of Applied Microbial Technology Sojo University
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MURAO SAWAO
Department of Applied Microbial Technology, Kumamoto Institute of Technology
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