Purification and Characterization of Thermostable Pectate Lyase with Protopectinase Activity from Thermophilic Bacillus sp. TS 47
スポンサーリンク
概要
- 論文の詳細を見る
A strain of thermophilic bacterium, Bacillus sp., with pectolytic activity has been isolated. It produced an extracellular endo-polygalacturonate trans-eliminase (PL, EC 4.2.2.1) when grown at 60℃ on a medium containing polygalacturonate (PGA). The PL was purified by hydrophobic, cation exchange, and size exclusion column chromatographies. The molecular mass of the enzyme was 50 kDa by SDS-PAGE. The isoelectric point of the enzyme was pH 5.3. The enzyme had a halflife of 13 and 1h at 65 and 70℃, respectively, and showed optimal activity around at 70℃ and pH 8.0. It had protopectinase activity, besides PL activity, on lemon protopectin and cotton fibers. The first 20 amino acids sequence of the enzyme had significant similarity with that of PL from methophilic Bacillus subtilis, with 50% identity.
- 社団法人日本農芸化学会の論文
- 2000-11-23
著者
-
Yoshikawa K
Laboratory Of Food Microbiological Engineering Faculty Of Agriculture Kinki University
-
Yoshikawa Kentaro
Laboratory Of Food Microbiological Engineering Faculty Of Agriculture Kinki University
-
TERASHITA Takao
Department of Food and Nutrition, Faculty of Agriculture, Kinki University
-
SAKAI TAKUO
Department of Agricultural Chemistry, College of Agriculture, University of Osaka Prefecture
-
Yoshikawa K
Department Of Bioscience Faculty Of Bioresources Mie University
-
TAKAO Makoto
Department of Food Science and Nutrition, Faculty of Agriculture, Kinki University
-
YOSHIKAWA Kentaro
Department of Food Science and Nutrition, Faculty of Agriculture, Kinki University
-
NAKANIWA Tetsuko
Department of Food Science and Nutrition, Faculty of Agriculture, Kinki University
-
Sakai Takuo
Laboratory Of Food Microbiological Engineering Faculty Of Agriculture Kinki University
-
Sakai Takuo
Fermentation Chemistry Laboratory College Of Agriculture University Of Osaka Prefecture
-
Sakai Takuo
Center For Research And Development Of Bioresources University Of Osaka Prefecture
-
Sakai Takuo
Department Of Food Science Faculty Of Agriculture Kinki University
-
Sakai Takuo
Department Of Applied Biological Chemistry College Of Agriculture University Of Osaka Prefecture
-
Sakai Takuo
Department Of Applied Biochemistry Faculty Of Agriculture Osaka Prefecture Univeisity
-
Sakai Takuo
Department Of Agricultural Chemistry College Of Agriculture University Of Osaka Prefecture
-
TAKAO Makoto
IGA Bioresearch
-
Takao Makoto
Laboratory Of Food Microbiological Engineering Department Of Food And Nutrition Faculty Of Agricultu
-
Takao Makoto
Department Of Agricultural Chemistry College Of Agriculture University Of Osaka Prefecture
-
Terashita T
Laboratory Of Food Microbiological Engineering Faculty Of Agriculture Kinki University
-
Terashita T
Kinki Univ. Nara Jpn
-
Terashita Takao
Department Of Agricultural Chemistry College Of Agriculture University Of Osaka Prefecture
-
Yoshikawa Kentaro
Department Of Food And Nutrition Faculty Of Agriculture Kinki University
-
Yoshida Kouji
Department Of Apple Research National Institute Of Fruit Tree Science
-
Nakaniwa Tetsuko
Department Of Food Science And Nutrition Faculty Of Agriculture Kinki University
関連論文
- A possible role of the key enzymes of the glyoxylate and gluconeogenesis pathways for fruit-body formation of the wood-rotting basidiomycete Flammulina velutipes
- Purification and Characterization of a Pectin-Releasing Enzyme Produced by Kluyveromyces wickerhamii
- Chromosomal Deletion or Rearrangement in Chimeric Hybrids of Saccharomycopsis fibuligera and Saccharomyces diastaticus Obtained by Cell Fusion
- Introduction of Mitochondria into Respiratory-Deficient Mutant of Yeast and Improvement of Thermostability
- Breeding a Fermentative Yeast at High Temperature Using Protoplast Fusion
- Purification and Properties of Cytosine Deaminase from Aspergillus fumigatus
- Production of Monoclonal Antibody against Protopectinase from Kluyveromyces wickerhamii : Studies of Enzymes Produced by Yeasts (IX).
- Characterization of HfO_2 Films Prepared on Various Surfaces for Gate Dielectrics(High-κ Gate Dielectrics)
- Characterization of HfO_2 Films Prepared on Various Surfaces for Gate Dielectrics
- Cloning and Heterologous Expression of Gene Encoding A Polygalacturonase from Aspergillus awamori
- Purification and Characterization of Acid-stable Protopectinase Produced by Aspergillus awamori in Solid-state Fermentation
- Horseradish Peroxidase Degrades Lipid Hydroperoxides and Suppresses Lipid Peroxidation of Polyunsaturated Fatty Acids in the Presence of Phenolic Antioxidants(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
- P-70(O-7) Development of new umu tester strain expressing human NADPH-P450 reductase and P450 1A2 involved in bioactivation of aromatic amines
- Purification and Characterization of an Endo-Polygalacturonase from a Mutant of Saccharomyces cerevisiae
- Isolation and Genetic Determination of a Saccharomyces cerevisiae Mutant That Produces an Endo-Polygalacturonase
- Molecular Cloning and Expression of a Polygalacturonase Gene in Saccharomyces cerevisiae
- Streptomyces Pepsin Inhibitor-insensitive Carboxyl Proteinase from Ganoderma lucidum
- Streptomyces Pepsin Inhibitor-Insensitive Carboxyl Proteinase from Lentinus edodes
- Purification and Some Properties of Carboxyl Proteinase in Mycelium of Lentinus edodes
- Promotive effect of the hot water-soluble fraction from corn fiber on vegetative mycelial growth in edible mushrooms
- Effect of trehalose on the spawn storage in some edible mushroom fungi (2): Effect on preservation in the freezer
- Molecular Cloning of the Gene Encoding Thermostable Endo-1,5-α-L-arabinase of Bacillus thermodenitrificans TS-3 and Its Expression in Bacillus subtilis(Biochemistry & Molecular Biology)
- Molecular Cloning, DNA Sequence, and Expression of the Gene Encoding for Thermostable Pectate Lyase of Thermophilic Bacillus sp. TS 47
- Purification and Characterization of Thermostable Pectate Lyase with Protopectinase Activity from Thermophilic Bacillus sp. TS 47
- Purification and Characterization of an Endo-Polygalacturonase from Aspergillus awamori
- Purification and Some Properties of Metallo Proteinase from the Pilei: Essential for the Growth of Pileus and Basidiospore Formation in the Fruit Body of Flammulina velutipes
- Studies on Protopectinase-C Mode of Action : Analysis of the Chemical Structure of the Specific Substrate in Sugar Beet Protopectin and Characterization of the Enzyme Activity
- Stabilization of Cytosine Deaminase from Bakers' Yeast by Immobilization(Microbiology & Fermentation Industry)
- Purification and Some Properties of Cytosine Deaminase from Bakers' Yeast(Microbiology & Fermentation Industry)
- Cytosine Deaminase from Escherichia coli : Production, Purification, and Some Characteristics(Microbiology & Fermentation Industry)
- Affinity Chromatography of Cytosine Deaminase from Escherichia coli with Immobilized Pyrimidine Compounds(Microbiology & Fermentation Industry)
- Visual Assay for Specific Inhibitors of Adenosine Deaminase with Agar Plates Containing pH Indicator
- Structural Basis for Thermostability of Endo-1,5-α-L-Arabinanase form Bacillus thermodenitrificans TS-3
- Inhibitory Effect of Phosphorylated Oligosaccharides Prepared from Potato Starch on the Formation of Calcium Phosphate
- Effective Production of Glycosyl-steviosides by α-1,6 Transglucosylation of Dextrin Dextranase
- Classification of Some α-Glucosidases and α-Xylosidases on the Basis of Substrate Specificity
- Substrate Specificity of Dextrin Dextranase from Acetobacter capsulatus ATCC 11894
- Transfer Action of α-D-Xylosidases from Aspergillus flavus MO-5 on p-Nitrophenyl-α-D-xylopyranoside
- Effective Dextran Production from Starch by Dextrin Dextranase with Debranching Enzyme
- Structure of Dextran Synthesized by Dextrin Dextranase from Acetobacter capsulatus ATCC 11894
- Purification and Characterization of an Intracellular α-D-Xylosidase II from Aspergillus flavus MO-5
- Isolation of Aspergillus flavus MO-5 Producing Two Types of Intracellular α-D-Xylosidases : Purification and Characterization of α-D-Xylosidase I
- Nucleotide Sequence of the Clostridium stercorarium xylA Gene Encoding a Bifunctional Protein with β-D-Xylosidase and α-L-Arabinofuranosidase Activities, and Properties of the Translated Product
- Dextran Synthesis from Reduced Maltooligosaccharides by Dextrin Dextranase from Acetobacter capsulatus ATCC 11894
- Detailed Action Mechanism of Dextrin Dextranase from Acetobacter capsulatus ATCC 11894
- Purification and Some Properties of Dextrin Dextranase from Acetobacter capsulatus ATCC 11894
- Cloning and Expression in Escherichia coli of Clostridium stercorarium Strain F-9 Genes Related to Xylan Hydrolysis(Microbiology & Fermentation Industry)
- ISOTOPE EFFECTS IN THE DYNAMICS OF DOUBLY EXCITED STATES OF MOLECULAR HYDROGEN AND DEUTERIUM AS STUDIED BY COINCIDENT ELECTRON-ENERGY-LOSS SPECTROSCOPY
- Cloning and Expression in Escherichia coli of Sucrose Phosphorylase Gene from Leuconostoc mesenteroides No. 165
- Screening for Bacteria Producing Sucrose Phosphorylase and Characterization of the Enzymes
- Cloning and Characterization of Zymomonas mobilis Genes Encoding Extracellular Levansucrase and Invertase
- Cloning and Characterization of a Pair of Genes That Stimulate the Production and Secretion of Zymomonas mobilis Extracellular Levansucrase and Invertase
- Purification and Characterization of Three Extracellular Protopectinases with Poly-galacturonase Activities from Trichosporon penicillatum
- Carboxyl Proteinase Essential for Maturation of Basidiospores of Lentinus edodes (shiitake)
- Enantioselective Oxidation of Diols by Secondary Alcohol Dehydrogenase from Geotrichum sp. WF9101
- Degradation of Vinyl Alcohol Oligomers by Geotrichum sp. WF9101
- Isolation and Characterization of a Strain of Bacillus megaterium That Degrades Poly(vinyl alcohol)
- Purification, Characterization, and Production of Two Pectic Transeliminases with Protopectinase Activity from Bacillus subtilis
- Purification and Properties of an S-PI(Pepstatin Ac)-insensitive Carboxyl Proteinase from a Xanthomonas sp. Bacterium(Biological Chemistry)
- Horizontal transmission and host-virulence attenuation of totivirus in violet root rot fungus Helicobasidium mompa
- Transmissibility of viral double-stranded RNA between strains of the violet root rot fungus Helicobasidium mompa and the potential for viral dsRNA infection to this fungus using monokaryotic strains
- Detection of tumorigenic Agrobacterium strains from infected apple saplings by colony PCR with improved PCR primers
- Purification and characterization of an extracellular acid trehalase from Lentinula edodes
- Purification and Some Properties of a Protopectin-solubilizing Enzyme that Has Potent Activity on Sugar Beet Protopectin(Biological Chemistry)
- DELETION AND RECOMBINATION OF PLASMID pU01 CODING FOR DEHALOGENATION OF HALOACETATE AND MERCURY RESISTANCE
- Purification and Characterization of Adenine Deaminase in Pseudomonas synxantha : Metabolism of nucleosides in bacteria (VIII)
- Purification, Crystallization, and Characterization of a Novel Protopectinase from Bacillus subtilis(Biological Chemistry)
- TREATMENT EFFECTS OF SKELETAL CLASS II CASES : DIFFERENCES IN TREATMENT TIMELINESS
- Microbial Metabolism of Nitriles in Pseudomonas sp. : Metabolism of Nitriles in Microorganisms. (VII)
- Protopectin Solubilizing Enzyme That Does Not Catalyze the Degradation of Polygalacturonic Acid(Biological Chemistry)
- Role of metal proteinases in the fruit-body formation of Hypsizygus marmoreus
- Isolation and characterization of extra- and intra-cellular metal proteinases produced in the spawn-running process of Hypsizygus marmoreus
- Productivity of hydrolytic enzymes by mycorrhizal mushrooms
- Purification and some properties of an isoform of metal proteinases from Hypsizygus marmoreus grown on sawdust culture
- Changes in carbohydrase activities during vegetative growth and development of fruit - bodies of Hypsizygus marmoreus grown in sawdustbased culture
- Desmutagenic Effect of Pheophytin from Japanese Eggplant against Several Mutagens
- Microbial Bioassay of Acute Toxicity by the pH Inhibition Method and Comparison of IC_ (pHI) with LD_ for Rats and Mice
- Inhibitory Effects of Whisky Polyphenols on Melanogenesis in Mouse B16 Melanoma Cells
- Use of Protoplast Fusion for the Development of Rapid Starch-fermenting Strains of Saccharomyces diastaticus
- Formation of β-Cyanoalanine by Cyanide-resistant Strain Enterobacter sp. 10-1
- Purification and Crystallization of a β-Cyanoalanineforming Enzyme from Enterobacter sp. 10-1
- Purification, Crystallization and Some Properties of Endopolygalacturonase from Kluyveromyces fragilis
- Purification and SomeProperties of a Protopectin-solubilizing Enzymefrom Galactomyces reessii Strain L
- Treatment of Bile Leaks from Cystohepatic and Common Hepatic Duct after Laparoscopic Cholecystectomy.
- Some Properties of a β-Cyanoalanine-forming Enzyme of Enterobacter sp. 10-1
- Purification, Crystallization and Some Properties of β-Cyano-L-alanine-degrading Enzyme in Pseudomonas sp. 13
- Possible Role of Cell Wall Mannan in the Secretion of the Protopectin-solubilizing Enzyme of Trichosporon penicillatum SNO-3
- Specificities of Bio-antimutagens in Plant Kingdom
- Microbial Utilization of β-Cyano-L-alanine by Pseudomonas sp. 13
- Percutaneous injection of adriamycin-lipiodol emulsion into hepatocellular carcinoma tumor nodules demonstrates lymph vessels connecting the tumor and the regional lymph nodes.
- Some Properties of Endo-polygalacturonase from Trichosporon penicillatum SNO-3
- Purification and Crystallization of a Protopectin-solubilizing Enzyme from Trichosporon penicillatum
- Desmutagenic Effect of Pheophytin b against Trp-P-2 in the Salmonella Assay.
- Purification and Some Properties of Metal Proteinases from Lentinus edodes
- Purification and Some Properties of Carboxyl Proteinase in Extract from Lentinus edodes Fruit-bodies