Purification and Characterization of Thermostable Pectate Lyase with Protopectinase Activity from Thermophilic Bacillus sp. TS 47
スポンサーリンク
概要
- 論文の詳細を見る
A strain of thermophilic bacterium, Bacillus sp., with pectolytic activity has been isolated. It produced an extracellular endo-polygalacturonate trans-eliminase (PL, EC 4.2.2.1) when grown at 60℃ on a medium containing polygalacturonate (PGA). The PL was purified by hydrophobic, cation exchange, and size exclusion column chromatographies. The molecular mass of the enzyme was 50 kDa by SDS-PAGE. The isoelectric point of the enzyme was pH 5.3. The enzyme had a halflife of 13 and 1h at 65 and 70℃, respectively, and showed optimal activity around at 70℃ and pH 8.0. It had protopectinase activity, besides PL activity, on lemon protopectin and cotton fibers. The first 20 amino acids sequence of the enzyme had significant similarity with that of PL from methophilic Bacillus subtilis, with 50% identity.
- 社団法人日本農芸化学会の論文
- 2000-11-23
著者
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Yoshikawa K
Laboratory Of Food Microbiological Engineering Faculty Of Agriculture Kinki University
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Yoshikawa Kentaro
Laboratory Of Food Microbiological Engineering Faculty Of Agriculture Kinki University
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TERASHITA Takao
Department of Food and Nutrition, Faculty of Agriculture, Kinki University
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SAKAI TAKUO
Department of Agricultural Chemistry, College of Agriculture, University of Osaka Prefecture
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Yoshikawa K
Department Of Bioscience Faculty Of Bioresources Mie University
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TAKAO Makoto
Department of Food Science and Nutrition, Faculty of Agriculture, Kinki University
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YOSHIKAWA Kentaro
Department of Food Science and Nutrition, Faculty of Agriculture, Kinki University
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NAKANIWA Tetsuko
Department of Food Science and Nutrition, Faculty of Agriculture, Kinki University
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Sakai Takuo
Laboratory Of Food Microbiological Engineering Faculty Of Agriculture Kinki University
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Sakai Takuo
Fermentation Chemistry Laboratory College Of Agriculture University Of Osaka Prefecture
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Sakai Takuo
Center For Research And Development Of Bioresources University Of Osaka Prefecture
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Sakai Takuo
Department Of Food Science Faculty Of Agriculture Kinki University
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Sakai Takuo
Department Of Applied Biological Chemistry College Of Agriculture University Of Osaka Prefecture
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Sakai Takuo
Department Of Applied Biochemistry Faculty Of Agriculture Osaka Prefecture Univeisity
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Sakai Takuo
Department Of Agricultural Chemistry College Of Agriculture University Of Osaka Prefecture
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TAKAO Makoto
IGA Bioresearch
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Takao Makoto
Laboratory Of Food Microbiological Engineering Department Of Food And Nutrition Faculty Of Agricultu
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Takao Makoto
Department Of Agricultural Chemistry College Of Agriculture University Of Osaka Prefecture
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Terashita T
Laboratory Of Food Microbiological Engineering Faculty Of Agriculture Kinki University
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Terashita T
Kinki Univ. Nara Jpn
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Terashita Takao
Department Of Agricultural Chemistry College Of Agriculture University Of Osaka Prefecture
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Yoshikawa Kentaro
Department Of Food And Nutrition Faculty Of Agriculture Kinki University
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Yoshida Kouji
Department Of Apple Research National Institute Of Fruit Tree Science
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Nakaniwa Tetsuko
Department Of Food Science And Nutrition Faculty Of Agriculture Kinki University
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