Affinity Chromatography of Cytosine Deaminase from Escherichia coli with Immobilized Pyrimidine Compounds(Microbiology & Fermentation Industry)
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概要
- 論文の詳細を見る
We immobilized many classes of pyrimidine compounds on the carrier Sepharose^[○!R] 4B via alkyl spacers by constructing various spacers using cyanogen bromide activation of the carrier. A crude enzyme solution of Escherichia coli with cytosine- and 5-fluorocytosine-deaminating activity was studied by adsorption and desorption chromatography with columns of the sixty-eight kinds of gels we made. Gels made with the following five ligands were effective. 2-Mercaptopyrimidine or 2-thiobarbituric acid, when coupled with 1,6-diaminohexane and then with the activated carrier, was suitable. So was 2-amino-4,6-dihydroxypyrimidine or 5-aminouracil, when linked by carbodiimide coupling to a carrier coupled with 6-aminohexanoic acid. Orotic acid, when linked in the same way with a carrier coupled with 1,4-diaminobutane, was also effective.
- 社団法人日本農芸化学会の論文
- 1986-07-23
著者
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KATSURAGI Tohoru
Graduate School of Biological Sciences, Nara Institute of Science and Technology
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SAKAI Takuo
Laboratory of Food Microbiological Engineering, Faculty of Agriculture Kinki University
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Sakai Takuo
Laboratory Of Food Microbiological Engineering Faculty Of Agriculture Kinki University
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Sakai Takuo
Fermentation Chemistry Laboratory College Of Agriculture University Of Osaka Prefecture
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Sakai Takuo
Center For Research And Development Of Bioresources University Of Osaka Prefecture
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Sakai Takuo
Department Of Food Science Faculty Of Agriculture Kinki University
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Sakai Takuo
Department Of Applied Biological Chemistry College Of Agriculture University Of Osaka Prefecture
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Sakai Takuo
Department Of Applied Biochemistry Faculty Of Agriculture Osaka Prefecture Univeisity
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Katsuragi Tohoru
Fermentation Chemistry Laboratory College Of Agriculture University Of Osaka Prefecture
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Katsuragi Tohoru
Laboratory Of Cell Biotechnology Graduate School Of Biological Sciences Nara Institute Of Science An
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Katsuragi Tohoru
Graduate School Of Biological Sciences Nara Institute Of Science And Technology (naist)
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TONOMURA Kenzo
Laboratory of Fermentation Chemistry, College of Agriculture, University of Osaka Prefecture
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Tonomura K
Department Of Food Technology Faculty Of Engineering Fukuyama University
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