Bacterial Communities in Petroleum Oil in Stockpiles (Environmental Biotechnology)
スポンサーリンク
概要
- 論文の詳細を見る
Bacterial communities in crude-oil samples from Japanese oil stockpiles were investigated by 16S rRNA gene cloning, followed by denaturing gradient gel electrophoresis (DGGE) analysis. 16S rRNA genes were successfully amplified by PCR after isooctane treatment from three kinds of crude-oil sample collected at four oil stockpiles in Japan. DGGE profiles showed that bacteria related to Ochrobactrum anthropi, Burkholderia cepacia, Stenotrophomonas maltophilia, Propionibacterium acnes, and Brevundimonas diminuta were frequently detected in most crude-oil samples. The bacterial communities differed in the sampling time and layer. Among the predominant bacteria detected in the crude oil, only three species were found for bacteria isolated on agar plates and were related to Burkholderia, Stenotrophomonas, and Propionibacterium, while Ochrobactrum sp. could not be isolated although this species seemed to be the most abundant bacterium in crude oil from the DGGE profiles. Using an archaea-specific primer set, methanogens were found in crude-oil sludge but not in crude-oil samples, indicating that methanogens might be involved in sludge formation in oil stockpiles.
- 社団法人日本生物工学会の論文
- 2005-02-25
著者
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Tani Yoshiki
Graduate School of Biological Sciences, Nara Institute of Science and Technology
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Yoshida N
Miyazaki Univ. Miyazaki Jpn
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Yoshida Naoto
Department Of Biological Resource Science Miyazaki University
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KATSURAGI Tohoru
Graduate School of Biological Sciences, Nara Institute of Science and Technology
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Yoshida Nobuyuki
Graduate School Of Biological Sciences Nara Institute Of Science And Technology
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Katsuragi Tohoru
Graduate School Of Biological Sciences Nara Institute Of Science And Technology
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KURANE Ryuichiro
Institute for Biological Resources and Functions, National Institute of Advanced Industrial, Science
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Tani Y
Graduate School Of Biological Sciences Nara Institute Of Science And Technology:department Of Biosci
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Tani Yoshiki
Graduate School Of Biological Sciences Nara Institute Of Science And Technology
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Yanagida Akira
Graduate School Of Biological Sciences Nara Institute Of Science And Technology
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Kanagawa T
Graduate School Of Biological Sciences Nara Institute Of Science And Technology
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SATO Daisuke
Graduate School of Science and Engineering, Aoyama Gakuin University
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YAGI KAZUHIRO
Graduate School of Biological Sciences, Nara Institute of Science and Technology
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WATANABE NORIKO
Graduate School of Biological Sciences, Nara Institute of Science and Technology
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KUROISHI TAKESHI
Graduate School of Biological Sciences, Nara Institute of Science and Technology
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NISHIMOTO KANA
Graduate School of Biological Sciences, Nara Institute of Science and Technology
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KANAGAWA TAKAHIRO
Institute of Biological Resources and Functions, National Institute of Advanced Industrial Science a
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Yagi Kazuhiro
Graduate School Of Biological Sciences Nara Institute Of Science And Technology
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Nishimoto Kana
Graduate School Of Biological Sciences Nara Institute Of Science And Technology
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Kanagawa Takahiro
Institute Of Biological Resources And Functions National Institute Of Advanced Industrial Science An
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Kanagawa Takahiro
Institute For Biological Resources And Functions National Institute Of Advanced Industrial Science A
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Tani Yoshiki
Graduate School Of Biological Sciences Advanced Institute Of Science And Technology
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Kuroishi T
Graduate School Of Biological Sciences Nara Institute Of Science And Technology
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Watanabe Noriko
Graduate School Of Biological Sciences Nara Institute Of Science And Technology
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Sato Daisuke
Graduate School Of Biological Sciences Nara Institute Of Science And Technology
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Kurane Ryuichiro
Institute Of Biological Resources And Functions National Institute Of Advanced Industrial Science And Technology
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Kurane Ryuichiro
Institute for Biological Resources and Functions, National Institute of Advanced Industrial Science and Technology (AIST)
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