Bias and Artifacts in Multitemplate Polymerase Chain Reactions (PCR)

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概要

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• Polymerase chain reaction (PCR) is often used for the amplification of a mixture of homologous genes. PCR bias and artifact formation can occur in multitemplate PCR, and provide incorrect information on the abundance and diversity of genes. PCR bias and artifact formation occur at a higher rate during the last few cycles of the reaction, and therefore can be avoided by stopping the PCR earlier.

• 公益社団法人日本生物工学会の論文
• 2003-10-25

著者

• Kanagawa Takahiro

  Institute For Biological Resources And Functions National Institute Of Advanced Industrial Science A

関連論文

• Bacterial Communities in Petroleum Oil in Stockpiles (Environmental Biotechnology)
• Bias and Artifacts in Multitemplate Polymerase Chain Reactions (PCR)

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