yaaD and yaaE Are Involved in Vitamin B_6 Biosynthesis in Bacillus subtilis
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概要
- 論文の詳細を見る
We show that yaaD and yaaE are involved in vitamin B_6 (B_6) biosynthesis in Bacillus subtilis. This is the first report which identifies genes involved in B_6 biosynthesis in B. subtilis. Based on homology, yaaD and yaaE belong to the highly conserved SNZ and SNO families, respectively. Disruptants of yaaD and yaaE required pyridoxal (PL) or pyridoxine (PN), and grew in the same way as the wild type in a minimal medium supplemented with 0.05 mM PL. The SNZ family is considered to be involved in singlet-oxygen resistance. Singlet-oxygen quenchers, L(+)-ascorbic acid and reduced glutathione, did not support the growth of these disruptants. Both yaaD and yaaE were transcribed at the highest level during the middle- to late-exponential phase and at a much lower level during the stationary phase. Neither PL nor PN affected the transcriptional rates of yaaD and yaaE. It is concluded that yaaD and yaaE are involved in B_6 biosynthesis in B. subtilis, and are transcribed at the highest level during the middle- to late-exponential phase.
- 社団法人日本生物工学会の論文
- 2002-03-25
著者
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Tani Yoshiki
Graduate School of Biological Sciences, Nara Institute of Science and Technology
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Sakai Akihiro
Graduate School Of Biological Sciences Nara Institute Of Science And Technology
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KATSURAGI Tohoru
Graduate School of Biological Sciences, Nara Institute of Science and Technology
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OGASAWARA Naotake
Graduate School of Biological Sciences, Nara Institute of Science and Technology
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Katsuragi Tohoru
Fermentation Chemistry Laboratory College Of Agriculture University Of Osaka Prefecture
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Katsuragi Tohoru
Graduate School Of Biological Sciences Nara Institute Of Science And Technology
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Katsuragi Tohoru
Graduate School Of Biological Sciences Nara Institute Of Science And Technology (naist)
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Kita Makoto
Graduate School Of Biological Sciences Nara Institute Of Science And Technology
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Tani Y
Graduate School Of Biological Sciences Nara Institute Of Science And Technology:department Of Biosci
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Tani Yoshiki
Graduate School Of Biological Sciences Nara Institute Of Science And Technology
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Ogasawara Naotake
Graduate School Of Biological Sciences Nara Institute Of Science And Technology
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Tani Yoshiki
Graduate School Of Biological Sciences Advanced Institute Of Science And Technology
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