Functional Analysis of the YvrGHb Two-Component System of Bacillus subtilis : Identification of the Regulated Genes by DNA Microarray and Northern Blot Analyses
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概要
- 論文の詳細を見る
YvrGHb is a novel two-component system in Bacillus subtilis. The yvrG and yvrHb genes are considered to encode sensor kinase and response regulator respectively. We found that the YvrGHb system positively regulates the 7 transcriptional units (wprA, wapA-yxxG, dltABCDE, sunA, sunT-bdbA-yolJ-bdbB, yvrI-yvrHa, and sigX-rsiX), and negatively regulates the lytABC operon. wprA, wapA, lytB, and lytC encode the main cell surface proteins of B. subtilis. Furthermore, SigX [extracytoplasmic function sigma factor (ECF sigma factor)] relating to the cell surface homeostatic functions, was regulated by this system. The yvrGHb null mutant showed the unusual autolysis and higher susceptibility to the four kinds of antibiotics (aztreonam, cefepime, bacitracin, and fosfomycin). These results indicate that the YvrGHb system is important to maintain the overall state of the cell surface through transcriptional regulation.
- 社団法人 日本農芸化学会の論文
- 2005-11-23
著者
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SERIZAWA Masakuni
Department of Gene Diagnostics and Therapeutics, Research Institute, International Medical Center of
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YAMAMOTO Hiroki
Department of Biochemistry, Osaka University of Pharmaceutical Sciences
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Kodama Keisuke
Department Of Applied Biology Faculty Of Textile Science And Technology Shinshu University
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OGASAWARA Naotake
Graduate School of Biological Sciences, Nara Institute of Science and Technology
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Sekiguchi Junichi
Department of Applied Biology, Faculty of Textile Science and Technology, Shinshu University
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Serizawa Masakuni
Department Of Applied Biology Faculty Of Textile Science And Technology Shinshu University
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Yamaguchi H
Department Of Applied Biology Faculty Of Textile Science And Technology Shinshu University
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KOBAYASHI Kazuo
Graduate School of Information Science, Nara Institute of Science and Technology
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Sekiguchi J
Department Of Applied Biology Faculty Of Textile Science And Technology Shinshu University
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Sekiguchi Junichi
Department Of Applied Biology Faculty Of Textile Science And Technology Shinshu University
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Ogasawara N
Graduate School Of Information Science Nara Institute Of Science And Technology
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Ogasawara Naotake
Graduate School Of Biological Sciences Nara Institute Of Science And Technology
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Yamamoto Hiroki
Department Of Agro-environmental Sciences Graduate School Of Bioresource And Bioenvironmental Scienc
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Yamamoto Hiroaki
Department Of Developmental Biology And Neurosciences Graduate School Of Life Sciences Tohoku Univer
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Yamamoto Hiroaki
Department Of Developmental Biology And Neurosciences Graduate School Of Life Sciences Tohoku Univer
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Kobayashi Kazuo
Graduate School Of Information Science Nara Institute Of Science And Technology
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