Overexpression, Purification, and Characteriaztion of Bacillus subtilis N-Acetylmuramoyl-L-alanine Amidase CwlC
スポンサーリンク
概要
- 論文の詳細を見る
N-acetylmuramoyl-L-alanine amidase CwlC of Bacillus subtilis was overproduced in Escherichia coli and purified 21-fold. The amidase hydrolyzed type A cell walls such as B. subtilis. The amidase bound slightly to the Microbacterium lacticum cell wall (type B), but did not entirely hydrolyze it. The presence of calcium or magnesium ion increased the resistance of the amidase to heat denaturation.
- 社団法人日本農芸化学会の論文
- 2000-07-23
著者
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SHIDA Toshio
Department of Applied Biology Faculty of Textile Science and Technology, Shinshu University
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Sekiguchi Junichi
Department of Applied Biology, Faculty of Textile Science and Technology, Shinshu University
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Shida Toshio
Department Of Applied Biology Faculty Of Textile Science And Technology Shinshu University
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HATTORI Hiromi
Department of Applied Biology, Faculty of Textile Science and Technology, Shinshu University
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ISE Fuminori
Department of Applied Biology, Faculty of Textile Science and Technology, Shinshu University
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Sekiguchi Junichi
Department Of Applied Biology Faculty Of Textile Science And Technology Shinshu University
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Ise Fuminori
Department Of Applied Biology Faculty Of Textile Science And Technology Shinshu University
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Hattori H
Department Of Applied Biology Faculty Of Textile Science And Technology Shinshu University
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