Genetic Mapping and Properties of Filamentous Mutations in Bacillus subtilis(Microbiology & Fermentation Industry)
スポンサーリンク
概要
- 論文の詳細を見る
Seven filamentous (fil) mutants were isolated from B, subtilis, and the mutations were mapped by means of lysed-protoplast transformation. Five of the mutations were linked to aroD and the others to pyrD, rgn mutations, which lead to a decrease in autolysin(s) and the formation of filaments, were also linked to aroD, and the mapping order was rgn-dnaE-aroD. On comparison with other reported filamentous mutations (lyt-1, lyt-2 and lyt-152), fil-1, fil-3 to -6, rgn and the above lyt mutations were determined to be in the same locus. All of the seven fil strains lacked flagella and showed decreased autolysin activity. Among them, only mutants having aroD-linked mutations showed low competency. Protease assay results indicated that rgn mutants produce a several times higher amount of the enzyme than the parent strain, and the initiation time for the production in rgn mutants was two hours earlier than in the parent strain.
- 社団法人日本農芸化学会の論文
- 1987-11-23
著者
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Akamatsu Takashi
Department of Applied Microbial Technology, Faculty of Biotechnology and Life Science, Sojo Universi
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Sekiguchi Junichi
Department of Applied Biology, Faculty of Textile Science and Technology, Shinshu University
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Akamatsu Takashi
Department Of Applied Microbial Technology Faculty Of Biotechnology And Life Science Sojo University
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Akamatsu T
Univ. Ryukyus Okinawa Jpn
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Sekiguchi Junichi
Department Of Applied Biology Faculty Of Textile Science And Technology Shinshu University
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Sekiguchi Junichi
Department Of Applied Biology Faculty Of Textile Science And Technology Shinsyu University
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