Characterization of a Polysaccharide Deacetylase Gene Homologue (pdaB) on Sporulation of Bacillus subtilis
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概要
- 論文の詳細を見る
The predicted amino acid sequence of Bacillus subtilis ybaN (renamed pdaB) exhibits high similarity to those of several polysaccharide deacetylases. Northern hybridization analysis with sporulation sigma mutants indicated that the pdaB gene is tran-scribed by EδE RNA polymerase and negatively regulated by SpoIIID. The pdaB mutant was deficient in spore formation. Phase- and electron microscopic observation showed morphological changes of spores in late sporulation periods. The pdaB spores that had lost their viability were empty. Moreover, GFP driven by the promoter of the sspE gene was localized in the forespore compartment for the wild type, but was localized in both the mother cell and forespore compartments for phase-gray/dark forespores of the pdaB mutant. This indicates that GFP expressed in the fore-spores of the mutant leaks into the mother cells. Therefore, PdaB is necessary to maintain spores after the late stage of sporulation.
- 社団法人 日本生化学会の論文
著者
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Sekiguchi Junichi
Department Of Applied Biology Faculty Of Textile Science And Technology Shinshu University
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Tanabe Taichi
Department Of Applied Biology Faculty Of Textile Science And Technology Shinshu University
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Hosoya Shigeo
International Environmental And Agricultural Science Tokyo University Of Agriculture And Technology
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Yamamoto Hiroki
Department Of Agro-environmental Sciences Graduate School Of Bioresource And Bioenvironmental Scienc
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Sato Tsutomu
International Environmental And Agricultural Science Tokyo University Of Agriculture And Technology
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Fukushima Tatsuya
Department Of Applied Biology Faculty Of Textile Science And Technology Shinshu University
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YOSHIKAWA Hirofumi
Department Bioscience and Biotechnology, Faculty of Agriculture, Graduate School, Kyushu University
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Tanabe Taichi
Departrnent of Applied Biology, Faculty of Textile Science and Technology, Shinshu University
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