Characterization of a New Bacillus subtilis Peptidoglycan Hydrolase Gene, yvcE (Named cwlO), and the Enzymatic Properties of Its Encoded Protein(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
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概要
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Bacillus subtilis yvcE (named cwlO) encodes a polypeptide consisting of 473 amino acid residues, and the N-terminal region contains a putative signal sequence and the C-terminal region exhibits high similarity to those of the NLPC/P60 superfamily (DL-endopeptidase family II). Northern blotting and cwlO-lacZ fusion analyses indicated that the cwlO gene is expressed as a monocistronic mRNA during the vegetative growth phase. The C-terminal region of CwlO was cloned into an Escherichia coli histidine-tagged vector and the protein, C-CwlO-6His, was produced in E. coil. The purified C-CwlO-6His protein exhibited cell wall hydrolase activity and the substrate bond specificity indicated that it is a DL-endopeptidase. The cell wall hydrolytic activity which seems to be derived from partially degraded CwlO (YvcE) was found in the culture supernatant of B. subtilis, but not in the cell wall binding fraction. The disruption of cwlO did not result in any difference in cell growth, morphology, or motility.
- 社団法人日本生物工学会の論文
- 2004-09-25
著者
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Yamaguchi Hiroyuki
Department of Internal Medicine, Yamagata Prefectural Shinjo Hospital
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YAMAMOTO Hiroki
Department of Biochemistry, Osaka University of Pharmaceutical Sciences
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Sekiguchi Junichi
Department of Applied Biology, Faculty of Textile Science and Technology, Shinshu University
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Yamaguchi Hiroyuki
Department Of Applied Biology Faculty Of Textile Science And Technology Shinshu University
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Yamaguchi H
Department Of Applied Biology Faculty Of Textile Science And Technology Shinshu University
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FUKUSHIMA Tatsuya
Department of Applied Biology, Faculty of Textile Science and Technology, Shinshu University
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FURUHATA KAZUMI
Department of Applied Biology, Faculty of Textile Science and Technology, Shinshu University
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Sekiguchi J
Department Of Applied Biology Faculty Of Textile Science And Technology Shinshu University
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Sekiguchi Junichi
Department Of Applied Biology Faculty Of Textile Science And Technology Shinshu University
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Furuhata Kazumi
Department Of Applied Biology Faculty Of Textile Science And Technology Shinshu University
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Yamamoto Hiroki
Department Of Agro-environmental Sciences Graduate School Of Bioresource And Bioenvironmental Scienc
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Yamamoto Hiroaki
Department Of Developmental Biology And Neurosciences Graduate School Of Life Sciences Tohoku Univer
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Yamamoto Hiroaki
Department Of Developmental Biology And Neurosciences Graduate School Of Life Sciences Tohoku Univer
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Fukushima Tatsuya
Department Of Applied Biology Faculty Of Textile Science And Technology Shinshu University
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