Purification and Characterization of Chitinase Isozymes from a Red Algae, Chondrus verrucosus
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概要
- 論文の詳細を見る
Three seaweed chitinase isozymes (Chi-A, B, and C) were purified from a red algae, Chondrus verrucosus. The molecular weights and isoelectric points were 24.5 kDa and 3.5 for Chi-A, 25.5 kDa and 4.6 for Chi-B, and 24.5 kDa and <3.5 for Chi-C. Optimum pH and temperature were observed at pH 2.0 at 80 °C for Chi-A and Chi-C, and at pH 1.0 and 70 °C for Chi-B. Toward N-acetylchitooligosaccharide (GlcNAcn) (n=2 to 6), Chi-A, B, and C hydrolyzed GlcNAc5 and GlcNAc6 and produced GlcNAcn (n=2 to 4). GlcNAcn (n=3, 4) with the reducing end-side of β anomer was detected in the hydrolysis products. These results indicate that the reactions of Chi-A, B, and C for GlcNAcn were a retaining mechanism similar to that of family 18 chitinase. Toward crystalline chitins, Chi-A, B, and C degraded squid pen β-chitin more than crab shell or shrimp shell α-chitin.
- 社団法人 日本農芸化学会の論文
- 2008-12-23
著者
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SATO Takuya
Department of Physics, Chuo University
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Sekiguchi Junichi
Department of Applied Biology, Faculty of Textile Science and Technology, Shinshu University
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MATSUMIYA Masahiro
Department of Marine Science and Resources, College of Bioresource Sciences, Nihon University
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Mochizuki Atsushi
College Of Bioresource Sciences Nihon University
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Miyauchi Kouji
College Of Bioresource Sciences Nihon University
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MIYAUCHI Kouji
Department of Marine Science and Resources, College of Bioresource Sciences, Nihon University
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MOCHIZUKI Atsushi
Department of Marine Science and Resources, College of Bioresource Sciences, Nihon University
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Miyauchi Kouji
Department Of Industrial Chemistry Faculty Of Engineering University Of Tokyo
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Mochizuki A
College Of Bioresource Sciences Nihon University
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Sekiguchi Junichi
Department Of Marine Science And Resources College Of Biosource Sciences Nihon University
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Sekiguchi Junichi
Department Of Applied Biology Faculty Of Textile Science And Technology Shinshu University
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Sato Takuya
Department Of Marine Science And Resources College Of Biosource Sciences Nihon University
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SHIROTA Kazuya
Department of Marine Science and Resources, College of Biosource Sciences, Nihon University
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Shirota Kazuya
Department Of Marine Science And Resources College Of Biosource Sciences Nihon University
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Matsumiya M
College Of Bioresource Sciences Nihon University
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Matsumiya Masahiro
Department Of Marine Science And Resources College Of Bioresource Sciences Nihon University
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Mochizuki Atsushi
Department Of Biology Faculty Of Science Kyushu University
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