Purification and Characterization of a 56 kDa Chitinase Isozyme (PaChiB) from the Stomach of the Silver Croaker, Pennahia argentatus

スポンサーリンク

概要

• 論文の詳細を見る

• A 56 kDa chitinase isozyme (PaChiB) was purified from the stomach of the silver croaker Pennahia argentatus. The optimum pH and pH stability of PaChiB were observed in an acidic pH range. When N-acetylchitooligosaccharides ((GlcNAc)n, n=2 –6) were used as substrates, PaChiB degraded (GlcNAc)4 –6 and produced (GlcNAc)2,3. It degraded (GlcNAc)5 to produce (GlcNAc)2 (23.2%) and (GlcNAc)3 (76.8%). The ability to degrade p-nitrophenyl N-acetylchitooligosaccharides (pNp-(GlcNAc)n, n=2 –4) fell in the following order: pNp-(GlcNAc)3 ≫ pNp-(GlcNAc)2 > pNp-(GlcNAc)4. Based on these results, we concluded that PaChiB is an endo-type chitinolytic enzyme, and that it preferentially hydrolyzes the third glycosidic bond from the non-reducing end of (GlcNAc)n. Activity toward crystalline α- and β-chitin was activated at 124%–185% in the presence of 0.5 M NaCl. PaChiB exhibited markedly high substrate specificity toward crab-shell α-chitin.

• Japan Society for Bioscience, Biotechnology, and Agrochemistryの論文

著者

• Miyauchi Kouji

  Department Of Industrial Chemistry Faculty Of Engineering University Of Tokyo

• Matsumiya Masahiro

  Department Of Marine Science And Resources College Of Bioresource Sciences Nihon University

• Miyauchi Kouji

  Department Of Marine Science And Resources College Of Biosource Sciences Nihon University

• IKEDA Mana

  Department of Marine Science and Resources, College of Biosource Sciences, Nihon University

関連論文

• Substrate Specificity of Chitinases from Two Species of Fish, Greenling, Hexagrammos otakii, and Common Mackerel, Scomber japonicus, and the Insect, Tobacco Hornworm, Manduca sexta
• Characterization of 38kDa and 42kDa chitinase isozymes from the liver of Japanese common squid Todarodes pacificus
• Nondestructive Evaluation for Distribution of Stress Using Photoacoustic Microscope : Photoacoustic Spectroscopy
• Purification and Characterization of Chitinase Isozymes from a Red Algae, Chondrus verrucosus
• Purification and some properties of a chitinase isozyme from the liver of Japanese common squid Todarodes pacificus
• Enzymatic Production of N-Acetyl-D-Glucosamine Using Crude Enzyme from the Liver of Squids
• Purification and Characterization of a 56kDa Chitinase Isozyme (PaChiB) from the Stomach of the Silver Croaker, Pennahia argentatus
• Purification and Characterization of a 56 kDa Chitinase Isozyme (PaChiB) from the Stomach of the Silver Croaker, Pennahia argentatus

スポンサーリンク